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. 1992 Oct;12(10):4297–4304. doi: 10.1128/mcb.12.10.4297

Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1.

D Ray 1, R Bosselut 1, J Ghysdael 1, M G Mattei 1, A Tavitian 1, F Moreau-Gachelin 1
PMCID: PMC360353  PMID: 1406622

Abstract

We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines.

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Selected References

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