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. 1992 Oct;12(10):4441–4448. doi: 10.1128/mcb.12.10.4441

Ectopic recombination between Ty elements in Saccharomyces cerevisiae is not induced by DNA damage.

A Parket 1, M Kupiec 1
PMCID: PMC360368  PMID: 1328855

Abstract

Mitotic recombination is increased when cells are treated with a variety of physical and chemical agents that cause damage to their DNA. We show here, using Saccharomyces cerevisiae strains that carry marked Ty elements, that recombination between members of this family of retrotransposons is not increased by UV irradiation or by treatment with the radiomimetic drug methyl methanesulfonate. Both ectopic recombination and mutation events were elevated by these agents for non-Ty sequences in the same strain. We discuss possible mechanisms that can prevent the induction of recombination between Ty elements.

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Selected References

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  1. Boeke J. D., Eichinger D., Castrillon D., Fink G. R. The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1. Mol Cell Biol. 1988 Apr;8(4):1432–1442. doi: 10.1128/mcb.8.4.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boeke J. D., Garfinkel D. J., Styles C. A., Fink G. R. Ty elements transpose through an RNA intermediate. Cell. 1985 Mar;40(3):491–500. doi: 10.1016/0092-8674(85)90197-7. [DOI] [PubMed] [Google Scholar]
  3. Boeke J. D., LaCroute F., Fink G. R. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984;197(2):345–346. doi: 10.1007/BF00330984. [DOI] [PubMed] [Google Scholar]
  4. Bradshaw V. A., McEntee K. DNA damage activates transcription and transposition of yeast Ty retrotransposons. Mol Gen Genet. 1989 Sep;218(3):465–474. doi: 10.1007/BF00332411. [DOI] [PubMed] [Google Scholar]
  5. Curcio M. J., Sanders N. J., Garfinkel D. J. Transpositional competence and transcription of endogenous Ty elements in Saccharomyces cerevisiae: implications for regulation of transposition. Mol Cell Biol. 1988 Sep;8(9):3571–3581. doi: 10.1128/mcb.8.9.3571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fabre F., Roman H. Genetic evidence for inducibility of recombination competence in yeast. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1667–1671. doi: 10.1073/pnas.74.4.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fleig U. N., Pridmore R. D., Philippsen P. Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae. Gene. 1986;46(2-3):237–245. doi: 10.1016/0378-1119(86)90408-7. [DOI] [PubMed] [Google Scholar]
  8. Friedberg E. C. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae. Microbiol Rev. 1988 Mar;52(1):70–102. doi: 10.1128/mr.52.1.70-102.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Giroux C. N., Mis J. R., Pierce M. K., Kohalmi S. E., Kunz B. A. DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae. Mol Cell Biol. 1988 Feb;8(2):978–981. doi: 10.1128/mcb.8.2.978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grimm C., Schaer P., Munz P., Kohli J. The strong ADH1 promoter stimulates mitotic and meiotic recombination at the ADE6 gene of Schizosaccharomyces pombe. Mol Cell Biol. 1991 Jan;11(1):289–298. doi: 10.1128/mcb.11.1.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jensen R., Sprague G. F., Jr, Herskowitz I. Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus. Proc Natl Acad Sci U S A. 1983 May;80(10):3035–3039. doi: 10.1073/pnas.80.10.3035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jinks-Robertson S., Petes T. D. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. doi: 10.1093/genetics/114.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Keil R. L., Roeder G. S. Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. cerevisiae. Cell. 1984 Dec;39(2 Pt 1):377–386. doi: 10.1016/0092-8674(84)90016-3. [DOI] [PubMed] [Google Scholar]
  15. Kupiec M., Petes T. D. Allelic and ectopic recombination between Ty elements in yeast. Genetics. 1988 Jul;119(3):549–559. doi: 10.1093/genetics/119.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kupiec M., Petes T. D. Meiotic recombination between repeated transposable elements in Saccharomyces cerevisiae. Mol Cell Biol. 1988 Jul;8(7):2942–2954. doi: 10.1128/mcb.8.7.2942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lichten M., Borts R. H., Haber J. E. Meiotic gene conversion and crossing over between dispersed homologous sequences occurs frequently in Saccharomyces cerevisiae. Genetics. 1987 Feb;115(2):233–246. doi: 10.1093/genetics/115.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lichten M., Haber J. E. Position effects in ectopic and allelic mitotic recombination in Saccharomyces cerevisiae. Genetics. 1989 Oct;123(2):261–268. doi: 10.1093/genetics/123.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Melamed C., Nevo Y., Kupiec M. Involvement of cDNA in homologous recombination between Ty elements in Saccharomyces cerevisiae. Mol Cell Biol. 1992 Apr;12(4):1613–1620. doi: 10.1128/mcb.12.4.1613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Montelone B. A., Prakash S., Prakash L. Recombination and mutagenesis in rad6 mutants of Saccharomyces cerevisiae: evidence for multiple functions of the RAD6 gene. Mol Gen Genet. 1981;184(3):410–415. doi: 10.1007/BF00352514. [DOI] [PubMed] [Google Scholar]
  21. Morawetz C., Hagen U. Effect of irradiation and mutagenic chemicals on the generation of ADH2- and ADH4-constitutive mutants in yeast: the inducibility of Ty transposition by UV and ethyl methanesulfonate. Mutat Res. 1990 Mar;229(1):69–77. doi: 10.1016/0027-5107(90)90009-s. [DOI] [PubMed] [Google Scholar]
  22. Petes T. D., Hill C. W. Recombination between repeated genes in microorganisms. Annu Rev Genet. 1988;22:147–168. doi: 10.1146/annurev.ge.22.120188.001051. [DOI] [PubMed] [Google Scholar]
  23. Picologlou S., Brown N., Liebman S. W. Mutations in RAD6, a yeast gene encoding a ubiquitin-conjugating enzyme, stimulate retrotransposition. Mol Cell Biol. 1990 Mar;10(3):1017–1022. doi: 10.1128/mcb.10.3.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Prakash L. Lack of chemically induced mutation in repair-deficient mutants of yeast. Genetics. 1974 Dec;78(4):1101–1118. doi: 10.1093/genetics/78.4.1101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ROMAN H., JACOB F. A comparison of spontaneous and ultraviolet-induced allelic recombination with reference to the recombination of outside markers. Cold Spring Harb Symp Quant Biol. 1958;23:155–160. doi: 10.1101/sqb.1958.023.01.019. [DOI] [PubMed] [Google Scholar]
  26. Rockmill B., Roeder G. S. Meiosis in asynaptic yeast. Genetics. 1990 Nov;126(3):563–574. doi: 10.1093/genetics/126.3.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rothstein R., Helms C., Rosenberg N. Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Mar;7(3):1198–1207. doi: 10.1128/mcb.7.3.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Simchen G. Are mitotic functions required in meiosis? Genetics. 1974 Apr;76(4):745–753. doi: 10.1093/genetics/76.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Simchen G., Kassir Y., Horesh-Cabilly O., Friedmann A. Elevated recombination and pairing structures during meiotic arrest in yeast of the nuclear division mutant cdc5. Mol Gen Genet. 1981;184(1):46–51. doi: 10.1007/BF00271193. [DOI] [PubMed] [Google Scholar]
  30. Simchen G., Winston F., Styles C. A., Fink G. R. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2431–2434. doi: 10.1073/pnas.81.8.2431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Simon J. R., Moore P. D. Induction of homologous recombination in Saccharomyces cerevisiae. Mol Gen Genet. 1988 Sep;214(1):37–41. doi: 10.1007/BF00340176. [DOI] [PubMed] [Google Scholar]
  32. Steele D. F., Morris M. E., Jinks-Robertson S. Allelic and ectopic interactions in recombination-defective yeast strains. Genetics. 1991 Jan;127(1):53–60. doi: 10.1093/genetics/127.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thomas B. J., Rothstein R. Elevated recombination rates in transcriptionally active DNA. Cell. 1989 Feb 24;56(4):619–630. doi: 10.1016/0092-8674(89)90584-9. [DOI] [PubMed] [Google Scholar]
  34. Vincent A., Petes T. D. Mitotic and meiotic gene conversion of Ty elements and other insertions in Saccharomyces cerevisiae. Genetics. 1989 Aug;122(4):759–772. doi: 10.1093/genetics/122.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Voelkel-Meiman K., Keil R. L., Roeder G. S. Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I. Cell. 1987 Mar 27;48(6):1071–1079. doi: 10.1016/0092-8674(87)90714-8. [DOI] [PubMed] [Google Scholar]
  36. WILKIE D., LEWIS D. THE EFFECT OF ULTRAVIOLET LIGHT ON RECOMBINATION IN YEAST. Genetics. 1963 Dec;48:1701–1716. doi: 10.1093/genetics/48.12.1701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Winston F., Chumley F., Fink G. R. Eviction and transplacement of mutant genes in yeast. Methods Enzymol. 1983;101:211–228. doi: 10.1016/0076-6879(83)01016-2. [DOI] [PubMed] [Google Scholar]

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