Figure 6.
Microparticles (MPs) generated using PPP method can be co-localized with dendritic cells (DCs) on a cellular array to quantify the effects of particles on DC functions in a high-throughput fashion. Particles encapsulating fluorescent dyes coumarin, rhodamine and cyanine were over-printed on an array using standard mini-arraying contact pin equipment and DCs were seeded on for 2 h. Individual spots of co-localized DCs and particles showing overlays of A.) coumarin dye (in blue) + cyanine dye (in red) = magenta; B.) coumarin dye (in blue) + rhodamine dye (in green) = cyan; demonstrating co-localization of particle formulations with cells.