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. 1990 May;10(5):2096–2103. doi: 10.1128/mcb.10.5.2096

Differential regulation of the N-myc gene in transfected cells and transgenic mice.

K Zimmerman 1, E Legouy 1, V Stewart 1, R Depinho 1, F W Alt 1
PMCID: PMC360557  PMID: 2183019

Abstract

The N-myc gene is expressed specifically in the early developmental stages of numerous cell lineages. To assay for sequences that could potentially regulate N-myc expression, we transfected constructs that contained murine N-myc genomic sequences linked to a reporter gene and genomic clones that contained the complete human or murine N-myc genes into cell lines that either express or do not express the endogenous N-myc gene. Following either transient or stable transfection, the introduced N-myc sequences were expressed regardless of the expression status of the endogenous gene. In contrast, when the clones containing the complete human N-myc gene were introduced into the germline of transgenic mice, expression in some transgenic lines paralleled the tissue- and stage-specific expression of the endogenous murine gene. These findings demonstrate differences in the regulation of N-myc genes in recipient cells following in vitro versus in vivo introduction, suggesting that early developmental events may play a role in the regulation of N-myc expression.

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Selected References

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