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. 1990 May;10(5):2308–2314. doi: 10.1128/mcb.10.5.2308

Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae.

T M Dunn 1, D Shortle 1
PMCID: PMC360578  PMID: 2183030

Abstract

Extragenic suppressors of a new temperature-sensitive mutation (act1-4) in the actin gene of Saccharomyces cerevisiae were isolated in an attempt to identify genes whose products interact directly with actin. One suppressor with a cold-sensitive growth phenotype defined the new gene, SAC7, which was mapped, cloned, sequenced, and disrupted. Genetic analysis of strains that are disrupted for SAC7 demonstrated that the protein is required for normal growth and actin assembly at low temperatures. Surprisingly, null mutations in SAC7 also suppressed the temperature-sensitive growth defect caused by the act1-1 and act1-4 mutations, whereas they were lethal in combination with the temperature-sensitive allele act1-2. These results support the notion that the SAC7 gene product is involved in the normal assembly or function or both of actin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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