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. 1990 Jun;10(6):3137–3144. doi: 10.1128/mcb.10.6.3137

Characterization of chicken c-ski oncogene products expressed by retrovirus vectors.

P Sutrave 1, T D Copeland 1, S D Showalter 1, S H Hughes 1
PMCID: PMC360678  PMID: 2188109

Abstract

We constructed replication-competent avian retrovirus vectors that contain two of the three known types of chicken c-ski cDNAs and a third vector that contains a truncated c-ski cDNA. We developed antisera that recognize the c-ski proteins made by the three transforming c-ski viruses. All three proteins (apparent molecular masses, 50, 60, and 90 kilodaltons) are localized primarily in the nucleus. The proteins are differentially phosphorylated; immunofluorescence also suggests that there are differences in subnuclear localization of the c-ski proteins and that c-ski protein is associated with condensed chromatin in dividing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barkas A. E., Brodeur D., Stavnezer E. Polyproteins containing a domain encoded by the V-SKI oncogene are located in the nuclei of SKV-transformed cells. Virology. 1986 May;151(1):131–138. doi: 10.1016/0042-6822(86)90111-x. [DOI] [PubMed] [Google Scholar]
  2. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  3. Colmenares C., Stavnezer E. The ski oncogene induces muscle differentiation in quail embryo cells. Cell. 1989 Oct 20;59(2):293–303. doi: 10.1016/0092-8674(89)90291-2. [DOI] [PubMed] [Google Scholar]
  4. Copeland T. D., Tsai W. P., Kim Y. D., Oroszlan S. Envelope proteins of human T cell leukemia virus type I: characterization by antisera to synthetic peptides and identification of a natural epitope. J Immunol. 1986 Nov 1;137(9):2945–2951. [PubMed] [Google Scholar]
  5. DeLorbe W. J., Luciw P. A., Goodman H. M., Varmus H. E., Bishop J. M. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J Virol. 1980 Oct;36(1):50–61. doi: 10.1128/jvi.36.1.50-61.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hughes S. H., Greenhouse J. J., Petropoulos C. J., Sutrave P. Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors. J Virol. 1987 Oct;61(10):3004–3012. doi: 10.1128/jvi.61.10.3004-3012.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Li Y., Turck C. M., Teumer J. K., Stavnezer E. Unique sequence, ski, in Sloan-Kettering avian retroviruses with properties of a new cell-derived oncogene. J Virol. 1986 Mar;57(3):1065–1072. doi: 10.1128/jvi.57.3.1065-1072.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Matsushima K., Kobayashi Y., Copeland T. D., Akahoshi T., Oppenheim J. J. Phosphorylation of a cytosolic 65-kDa protein induced by interleukin 1 in glucocorticoid pretreated normal human peripheral blood mononuclear leukocytes. J Immunol. 1987 Nov 15;139(10):3367–3374. [PubMed] [Google Scholar]
  9. Nagase T., Mizuguchi G., Nomura N., Ishizaki R., Ueno Y., Ishii S. Requirement of protein co-factor for the DNA-binding function of the human ski proto-oncogene product. Nucleic Acids Res. 1990 Jan 25;18(2):337–343. doi: 10.1093/nar/18.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nomura N., Sasamoto S., Ishii S., Date T., Matsui M., Ishizaki R. Isolation of human cDNA clones of ski and the ski-related gene, sno. Nucleic Acids Res. 1989 Jul 25;17(14):5489–5500. doi: 10.1093/nar/17.14.5489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spector D. L., Watt R. A., Sullivan N. F. The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles. Oncogene. 1987 Mar;1(1):5–12. [PubMed] [Google Scholar]
  13. Stavnezer E., Barkas A. E., Brennan L. A., Brodeur D., Li Y. Transforming Sloan-Kettering viruses generated from the cloned v-ski oncogene by in vitro and in vivo recombinations. J Virol. 1986 Mar;57(3):1073–1083. doi: 10.1128/jvi.57.3.1073-1083.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stavnezer E., Brodeur D., Brennan L. A. The v-ski oncogene encodes a truncated set of c-ski coding exons with limited sequence and structural relatedness to v-myc. Mol Cell Biol. 1989 Sep;9(9):4038–4045. doi: 10.1128/mcb.9.9.4038. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stavnezer E., Gerhard D. S., Binari R. C., Balazs I. Generation of transforming viruses in cultures of chicken fibroblasts infected with an avian leukosis virus. J Virol. 1981 Sep;39(3):920–934. doi: 10.1128/jvi.39.3.920-934.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sutrave P., Hughes S. H. Isolation and characterization of three distinct cDNAs for the chicken c-ski gene. Mol Cell Biol. 1989 Sep;9(9):4046–4051. doi: 10.1128/mcb.9.9.4046. [DOI] [PMC free article] [PubMed] [Google Scholar]

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