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. 1990 Jul;10(7):3325–3333. doi: 10.1128/mcb.10.7.3325

Molecular organization of the human Raf-1 promoter region.

T W Beck 1, U Brennscheidt 1, G Sithanandam 1, J Cleveland 1, U R Rapp 1
PMCID: PMC360752  PMID: 1694010

Abstract

A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had features of a housekeeping gene in that it was GC-rich (HTF-like), lacked TATA and CAAT boxes, and contained heterogeneous RNA start sites and four potential binding sites for the transcription factor SP1. In addition, an octamer motif (ATTTCAT), a potential binding site for the octamer family of transcription factors, was located at -734 base pairs. The Raf-1 promoter region drove reporter gene expression 30-fold over the promoterless reporter in Cos 7 cells.

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Selected References

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