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. Author manuscript; available in PMC: 2013 Dec 21.
Published in final edited form as: Biochemistry. 2012 Dec 11;51(51):10218–10228. doi: 10.1021/bi301287h

Figure 2.

Figure 2

Kinase activity as array ages. Serine receptor-containing E. coli membranes were reconstituted in vitro with Cysless CheA kinase and CheW coupling protein to yield triplicate arrays, then were incubated at 22°C for 504 hr (21 days). At the indicated timepoints a sample was removed from each triplicate and mixed with CheY and ATP to measure the array kinase activity (closed circles). An identical sample was mixed with attractant serine to measure attractant regulation (open circles). The data were best fit either with the double exponential decay of Eq. 1 (curve, no serine) or with a straight line (serine). Error bars indicate the standard deviation of each triplicate mean.