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. 1990 Jul;10(7):3828–3833. doi: 10.1128/mcb.10.7.3828

Induction of transformation and DNA synthesis after microinjection of raf proteins.

M R Smith 1, G Heidecker 1, U R Rapp 1, H F Kung 1
PMCID: PMC360849  PMID: 2192265

Abstract

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3 cells induced DNA synthesis and morphological transformation, whereas microinjection of full-length c-raf had no effect. The amino terminus of the raf protein has an important negative regulatory influence; alteration of this region resulted in increased kinase activity and oncogenicity.

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Selected References

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