Abstract
Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UASRPG elements). We found that UASRPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1, TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity.
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- Baker H. V. Glycolytic gene expression in Saccharomyces cerevisiae: nucleotide sequence of GCR1, null mutants, and evidence for expression. Mol Cell Biol. 1986 Nov;6(11):3774–3784. doi: 10.1128/mcb.6.11.3774. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buchman A. R., Lue N. F., Kornberg R. D. Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein. Mol Cell Biol. 1988 Dec;8(12):5086–5099. doi: 10.1128/mcb.8.12.5086. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Capieaux E., Vignais M. L., Sentenac A., Goffeau A. The yeast H+-ATPase gene is controlled by the promoter binding factor TUF. J Biol Chem. 1989 May 5;264(13):7437–7446. [PubMed] [Google Scholar]
- Chambers A., Stanway C., Kingsman A. J., Kingsman S. M. The UAS of the yeast PGK gene is composed of multiple functional elements. Nucleic Acids Res. 1988 Sep 12;16(17):8245–8260. doi: 10.1093/nar/16.17.8245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clifton D., Fraenkel D. G. The gcr (glycolysis regulation) mutation of Saccharomyces cerevisiae. J Biol Chem. 1981 Dec 25;256(24):13074–13078. [PubMed] [Google Scholar]
- Cohen R., Yokoi T., Holland J. P., Pepper A. E., Holland M. J. Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences. Mol Cell Biol. 1987 Aug;7(8):2753–2761. doi: 10.1128/mcb.7.8.2753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cottrelle P., Cool M., Thuriaux P., Price V. L., Thiele D., Buhler J. M., Fromageot P. Either one of the two yeast EF-1 alpha genes is required for cell viability. Curr Genet. 1985;9(8):693–697. doi: 10.1007/BF00449823. [DOI] [PubMed] [Google Scholar]
- Cottrelle P., Thiele D., Price V. L., Memet S., Micouin J. Y., Marck C., Buhler J. M., Sentenac A., Fromageot P. Cloning, nucleotide sequence, and expression of one of two genes coding for yeast elongation factor 1 alpha. J Biol Chem. 1985 Mar 10;260(5):3090–3096. [PubMed] [Google Scholar]
- Hamil K. G., Nam H. G., Fried H. M. Constitutive transcription of yeast ribosomal protein gene TCM1 is promoted by uncommon cis- and trans-acting elements. Mol Cell Biol. 1988 Oct;8(10):4328–4341. doi: 10.1128/mcb.8.10.4328. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herruer M. H., Mager W. H., Woudt L. P., Nieuwint R. T., Wassenaar G. M., Groeneveld P., Planta R. J. Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift. Nucleic Acids Res. 1987 Dec 23;15(24):10133–10144. doi: 10.1093/nar/15.24.10133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holland M. J., Yokoi T., Holland J. P., Myambo K., Innis M. A. The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Feb;7(2):813–820. doi: 10.1128/mcb.7.2.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huet J., Cottrelle P., Cool M., Vignais M. L., Thiele D., Marck C., Buhler J. M., Sentenac A., Fromageot P. A general upstream binding factor for genes of the yeast translational apparatus. EMBO J. 1985 Dec 16;4(13A):3539–3547. doi: 10.1002/j.1460-2075.1985.tb04114.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huet J., Sentenac A. TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3648–3652. doi: 10.1073/pnas.84.11.3648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larkin J. C., Thompson J. R., Woolford J. L., Jr Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene. Mol Cell Biol. 1987 May;7(5):1764–1775. doi: 10.1128/mcb.7.5.1764. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leer R. J., Van Raamsdonk-Duin M. M., Mager W. H., Planta R. J. Conserved sequences upstream of yeast ribosomal protein genes. Curr Genet. 1985;9(4):273–277. doi: 10.1007/BF00419955. [DOI] [PubMed] [Google Scholar]
- Machida M., Uemura H., Jigami Y., Tanaka H. The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene. Nucleic Acids Res. 1988 Feb 25;16(4):1407–1422. doi: 10.1093/nar/16.4.1407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mager W. H. Control of ribosomal protein gene expression. Biochim Biophys Acta. 1988 Jan 25;949(1):1–15. doi: 10.1016/0167-4781(88)90048-6. [DOI] [PubMed] [Google Scholar]
- Ogden J. E., Stanway C., Kim S., Mellor J., Kingsman A. J., Kingsman S. M. Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences. Mol Cell Biol. 1986 Dec;6(12):4335–4343. doi: 10.1128/mcb.6.12.4335. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Planta R. J., Raué H. A. Control of ribosome biogenesis in yeast. Trends Genet. 1988 Mar;4(3):64–68. doi: 10.1016/0168-9525(88)90042-x. [DOI] [PubMed] [Google Scholar]
- Rotenberg M. O., Woolford J. L., Jr Tripartite upstream promoter element essential for expression of Saccharomyces cerevisiae ribosomal protein genes. Mol Cell Biol. 1986 Feb;6(2):674–687. doi: 10.1128/mcb.6.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Runge K. W., Zakian V. A. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol Cell Biol. 1989 Apr;9(4):1488–1497. doi: 10.1128/mcb.9.4.1488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Santangelo G. M., Tornow J., McLaughlin C. S., Moldave K. Properties of promoters cloned randomly from the Saccharomyces cerevisiae genome. Mol Cell Biol. 1988 Oct;8(10):4217–4224. doi: 10.1128/mcb.8.10.4217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shore D., Nasmyth K. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements. Cell. 1987 Dec 4;51(5):721–732. doi: 10.1016/0092-8674(87)90095-x. [DOI] [PubMed] [Google Scholar]
- Stanway C., Mellor J., Ogden J. E., Kingsman A. J., Kingsman S. M. The UAS of the yeast PGK gene contains functionally distinct domains. Nucleic Acids Res. 1987 Sep 11;15(17):6855–6873. doi: 10.1093/nar/15.17.6855. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uemura H., Shiba T., Paterson M., Jigami Y., Tanaka H. Identification of a sequence containing the positive regulatory region of Saccharomyces cerevisiae gene ENO1. Gene. 1986;45(1):67–75. doi: 10.1016/0378-1119(86)90133-2. [DOI] [PubMed] [Google Scholar]