Figure 1. Predicted protein sequence of the sGAI gene of A. awamori (GenBank:JX559863) expressed in S. cerevisiae Y294[ySYAG]. The XYNSEC secretion signal is indicated in bold font. The sequence identified in glucoamylases essential for raw starch hydrolysis was conserved (PL(W-597)YVTVTLPA),¹⁹ as well as the second tryptophan (Trp) residue and is double underlined in gray text. The Gp-I region is indicated as text in italics bold. The Cp-I region or Starch Binding Domain is indicated in underlined text.²⁵ The general acid and base catalysts Glu-213 and Glu-434, as well as Tyr-85, Trp-87, Arg-89, Asp-90, Trp-154, Glu-214, Arg-339, Asp-343, Trp-351 sites, which play a role in substrate transition-state stabilization and/or ground-state binding, are indicated in gray text. Likely N-glycosylation sites are underlined by a broken line, although only the first and third sites were found to be glycosylated when expressed in yeast.²⁶