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. 1990 Mar;10(3):1287–1292. doi: 10.1128/mcb.10.3.1287

Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

V K Parnaik 1, P K Kennady 1
PMCID: PMC361022  PMID: 2137554

Abstract

A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.

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