Figure 2. Expression of miR156.
(A and B) Accumulation of miR156 in 8-, 9-, and 16-day-old long day plants. Expression of miR156 was analyzed by small RNA blot (A) and qRT-PCR (B). The plants were collected at Zeitgeber time (ZT) 24. The expression level of miR156 in 8-day-old seedlings was set to 1. (C and E) Expression of pri-MIR156A (C) and pri-MIR156C (E). The plants were collected every 4 hr and subjected to qRT-PCR analyses. Black and white boxes indicate dark and light conditions, respectively. (D and F) Expression of pri-MIR156A (D) and pri-MIR156C (F) during the shift from long day (LD) to constant light (CL) conditions. Five-day-old wild type seedlings were shifted from long day to constant light conditions. The seedlings were collected at ZT 16, 24, and 32.
Figure 2—figure supplement 1. Expression pattern of miR156.
Expression of miR156. The plants were collected every 4 hr and subjected to qRT-PCR analyses. ZT: Zeitgeber time.