Table 1.
Strains used to study histone ubiquitylation and sumoylation
| Strain | Genotype | Source |
|---|---|---|
| YZS276 |
MATa, hta1-htb1∷LEU2, HTA2-GAL1/GAL10-HTB2, ura3-1, trp1-1, leu2-3,-112, his3-11, ade2-1, can1-100 <pZS145 HTA1-Flag-HTB1,CEN, HIS3> |
[5] |
| YZS277 |
MATa, hta1-htb1∷LEU2, HTA2-GAL1/GAL10-HTB2, ura3-1, trp1-1, leu2-3,-112, his3-11, ade2-1, can1-100 <pZS146 HTA1-Flag-htb1-K123R, CEN,HIS3> |
[5] |
| YSN545 | MATa (hta1-htb1)Δ∷LEU2, (hta2-htb2) Δ∷TRP1, his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2-128Δ <pZS145 HTA1-Flag-HTB1-HIS3> |
[26] |
| YSN763 | MATa (hta1-htb1)Δ∷LEU2, (hta2-htb2) Δ∷TRP1, his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2-128Δ <pZS145 HTA1-Flag- htb1K123A-HIS3> |
[26] |
| YSF200 | MATa his3 1 leu2 0 met15 0 ura3 hhf2-hht2∷NAT hta1- htb1∷HPH hht1-hht2∷KAN hta2-htb2∷NAT <pRS315- HHT2-HHF2-HTA1-Flag-HTB1> |
[26] |
| YSF201 | MATa his3 1 leu2 0 met15 0 ura3 hhf2-hht2∷NAT hta1- htb1∷HPH hht1-hht2∷KAN hta2-htb2∷NAT <pRS315- HHT2-HHF2-HTA1-Flag-htb1K123R> |
[26] |
| CFK920 |
MATa, hta1-htb1∷LEU2, hta2-htb2, ura3-1, trp1-1, leu2-3,- 112, his3-11, ade2-1, can1-100, GAPDH-HA-UBI4∷URA3 <pZS145 HTA1-Flag-HTB1, CEN, HIS3> |
[17] |
| CFK921 |
MATa, hta1-htb1∷LEU2, hta2-htb2, ura3-1, trp1-1, leu2-3,-112 his3-11, ade2-1, can1-100, GAPDH-HA-UBI4∷URA3 <pZS146 HTA1-Flag-htb1-K123R, CEN, HIS3> |
[17] |
|
Flag H2A strain |
MATa (hta1-htb1)Δ∷LEU2, (hta2-htb2)Δ∷TRP1, his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2-128Δ <pRS315 (Flag-HTA1- HTB1)> |
[18] |
|
Flag H3 strain |
MATa his3Δ200 leu2 Δ 1 ura3-52 trp1Δ63 lys2-128Δ (hht1- hhf1) Δ∷LEU2 (hht2-hhf2) Δ∷HIS3< pRM204 (FlagHHT1- HHF1)> |
[51] |
|
Flag H4 strain |
MATa his3Δ200 leu2 Δ 1 ura3-52 trp1Δ63 lys2-128Δ (hht1- hhf1) Δ∷LEU2 (hht2-hhf2) Δ∷HIS3 <pRS426 (HHT1-Flag- HHF1)> |
[18] |
YZS276 and YZS277 are based on the Y131 shuffle strain, which is in the W303 background [10]. YSN545 and YSN763 were generated following plasmid shuffling from FY406, which is in the S288c background [52]. YSF200 and YSF201 are based on the quadruple histone deletion strain, JHY205, also in the S288c background [53]. CFK920 and CFK921 are used for double ChIP analysis and were made by transforming YZS276 and YZS277 with an integrating URA3 plasmid containing HA tagged ubiquitin under control of the constitutive GAPDH promoter. The Flag tagged H2A, H3 and H4 strains were made by plasmid shuffling from the FY1716 strain, also in the S288c background [51].