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. 1990 Sep;10(9):4967–4969. doi: 10.1128/mcb.10.9.4967

Oxidant stress leads to transcriptional activation of the human heme oxygenase gene in cultured skin fibroblasts.

S M Keyse 1, L A Applegate 1, Y Tromvoukis 1, R M Tyrrell 1
PMCID: PMC361123  PMID: 2388632

Abstract

Treatment of cultured human skin fibroblasts with near-UV radiation, hydrogen peroxide, and sodium arsenite induces accumulation of heme oxygenase mRNA and protein. In this study, these treatments led to a dramatic increase in the rate of RNA transcription from the heme oxygenase gene but had no effect on mRNA stability. Transcriptional activation, therefore, appears to be the major mechanism of stimulation of expression of this gene by either oxidative stress or sulfydryl reagents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alam J., Shibahara S., Smith A. Transcriptional activation of the heme oxygenase gene by heme and cadmium in mouse hepatoma cells. J Biol Chem. 1989 Apr 15;264(11):6371–6375. [PubMed] [Google Scholar]
  2. Christman M. F., Morgan R. W., Jacobson F. S., Ames B. N. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium. Cell. 1985 Jul;41(3):753–762. doi: 10.1016/s0092-8674(85)80056-8. [DOI] [PubMed] [Google Scholar]
  3. Demple B., Halbrook J. Inducible repair of oxidative DNA damage in Escherichia coli. Nature. 1983 Aug 4;304(5925):466–468. doi: 10.1038/304466a0. [DOI] [PubMed] [Google Scholar]
  4. Groudine M., Peretz M., Weintraub H. Transcriptional regulation of hemoglobin switching in chicken embryos. Mol Cell Biol. 1981 Mar;1(3):281–288. doi: 10.1128/mcb.1.3.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gutteridge J. M., Smith A. Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation. Biochem J. 1988 Dec 15;256(3):861–865. doi: 10.1042/bj2560861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gutteridge J. M. The antioxidant activity of haptoglobin towards haemoglobin-stimulated lipid peroxidation. Biochim Biophys Acta. 1987 Feb 14;917(2):219–223. doi: 10.1016/0005-2760(87)90125-1. [DOI] [PubMed] [Google Scholar]
  7. Ishizawa S., Yoshida T., Kikuchi G. Induction of heme oxygenase in rat liver. Increase of the specific mRNA by treatment with various chemicals and immunological identity of the enzymes in various tissues as well as the induced enzymes. J Biol Chem. 1983 Apr 10;258(7):4220–4225. [PubMed] [Google Scholar]
  8. Keyse S. M., Tyrrell R. M. Both near ultraviolet radiation and the oxidizing agent hydrogen peroxide induce a 32-kDa stress protein in normal human skin fibroblasts. J Biol Chem. 1987 Oct 25;262(30):14821–14825. [PubMed] [Google Scholar]
  9. Keyse S. M., Tyrrell R. M. Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite. Proc Natl Acad Sci U S A. 1989 Jan;86(1):99–103. doi: 10.1073/pnas.86.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keyse S. M., Tyrrell R. M. Induction of the heme oxygenase gene in human skin fibroblasts by hydrogen peroxide and UVA (365 nm) radiation: evidence for the involvement of the hydroxyl radical. Carcinogenesis. 1990 May;11(5):787–791. doi: 10.1093/carcin/11.5.787. [DOI] [PubMed] [Google Scholar]
  11. Kikuchi G., Yoshida T. Function and induction of the microsomal heme oxygenase. Mol Cell Biochem. 1983;53-54(1-2):163–183. doi: 10.1007/BF00225252. [DOI] [PubMed] [Google Scholar]
  12. Maines M. D., Kappas A. Studies on the mechanism of induction of haem oxygenase by cobalt and other metal ions. Biochem J. 1976 Jan 15;154(1):125–131. doi: 10.1042/bj1540125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maines M. D. New developments in the regulation of heme metabolism and their implications. Crit Rev Toxicol. 1984;12(3):241–314. doi: 10.3109/10408448409021604. [DOI] [PubMed] [Google Scholar]
  14. Müller R. M., Taguchi H., Shibahara S. Nucleotide sequence and organization of the rat heme oxygenase gene. J Biol Chem. 1987 May 15;262(14):6795–6802. [PubMed] [Google Scholar]
  15. Shibahara S., Müller R. M., Taguchi H. Transcriptional control of rat heme oxygenase by heat shock. J Biol Chem. 1987 Sep 25;262(27):12889–12892. [PubMed] [Google Scholar]
  16. Shibahara S., Sato M., Muller R. M., Yoshida T. Structural organization of the human heme oxygenase gene and the function of its promoter. Eur J Biochem. 1989 Feb 15;179(3):557–563. doi: 10.1111/j.1432-1033.1989.tb14583.x. [DOI] [PubMed] [Google Scholar]
  17. Stocker R., Yamamoto Y., McDonagh A. F., Glazer A. N., Ames B. N. Bilirubin is an antioxidant of possible physiological importance. Science. 1987 Feb 27;235(4792):1043–1046. doi: 10.1126/science.3029864. [DOI] [PubMed] [Google Scholar]
  18. Tyrrell R. M., Pidoux M. Quantitative differences in host cell reactivation of ultraviolet-damaged virus in human skin fibroblasts and epidermal keratinocytes cultured from the same foreskin biopsy. Cancer Res. 1986 Jun;46(6):2665–2669. [PubMed] [Google Scholar]
  19. Yoshida T., Biro P., Cohen T., Müller R. M., Shibahara S. Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem. 1988 Feb 1;171(3):457–461. doi: 10.1111/j.1432-1033.1988.tb13811.x. [DOI] [PubMed] [Google Scholar]

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