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. 1990 Sep;10(9):4987–4989. doi: 10.1128/mcb.10.9.4987

Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation.

J Singer-Sam 1, M Grant 1, J M LeBon 1, K Okuyama 1, V Chapman 1, M Monk 1, A D Riggs 1
PMCID: PMC361130  PMID: 1697035

Abstract

A HpaII-PCR assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is less than or equal to 10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days; about the time of X-chromosome inactivation of the inner cell mass.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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