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. 1990 Oct;10(10):5359–5364. doi: 10.1128/mcb.10.10.5359

Transformation of mouse BALB 3T3 cells by enterobacterial plasmid misrepair gene mucAB.

M Tosu 1, H Tanooka 1
PMCID: PMC361233  PMID: 2398894

Abstract

The enterobacterial plasmid misrepair gene mucAB, ligated to the metal-inducible mammalian MT-1 promoter, was introduced into the genome of mouse BALB 3T3 cells. In the presence of zinc ions, MucA but not MucB protein was produced, and the whole-cell population of each mucAB+ clone started to show the transformation phenotype in a few days. Foci appeared in the transformed cell population after 4 weeks, and cells from the foci produced tumors in nude mice, indicating malignant transformation by the mucA product. Growth of mucAB+ cells was stimulated by zinc-induced expression of mucA. The transformation phenotype was reversed by removing zinc ions from the culture, indicating that the transformation was due not to MucA-mediated mutation in the mouse genome but to the direct transforming activity of MucA protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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