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. 1990 Oct;10(10):5575–5579. doi: 10.1128/mcb.10.10.5575

RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.

H N Wu 1, M M Lai 1
PMCID: PMC361278  PMID: 2398903

Abstract

Hepatitis delta virus (HDV) RNA subfragments undergo self-cleavage at varying efficiencies. We have developed a procedure of using repeated cycles of heat denaturation and renaturation of RNA to achieve a high efficiency of cleavage. This effect can also be achieved by gradual denaturation of RNA with heat or formamide. These results suggest that only a subpopulation of the catalytic RNA molecules assumes the active conformation required for self-cleavage. This procedure could be of general use for detecting catalytic RNA activities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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