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. 1990 Apr;1(5):425–434. doi: 10.1091/mbc.1.5.425

Regulation of IL-4 lymphokine gene expression and cellular proliferation in murine T helper type II cells.

E Muñoz 1, A M Zubiaga 1, J Muñoz 1, B T Huber 1
PMCID: PMC361522  PMID: 1983070

Abstract

Activation of T cells results in the production of lymphokines and cellular proliferation. Protein kinase C (PKC) plays a key role in this process. It has been shown that this enzyme is essential to elicit a response to Con A or specific antigen in CD4+ T helper type 1 (Th 1) cells that secrete IL-2. We have now explored the signal transduction pathway that leads to transcription of the IL-4 gene and proliferation in murine CD4+ T helper type 2 (Th 2) cells. Surprisingly, we have found in two independently derived Th 2 clones that neither cellular proliferation nor IL-4 lymphokine production is affected by blocking or depletion of PKC. This differential mechanism of signal transmission leading to cellular activation implies a new distinction between murine Th 1 and Th 2 cells.

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