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. 1990 May;1(6):465–469. doi: 10.1091/mbc.1.6.465

Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor.

F Shilling 1, G Mandel 1, L A Jaffe 1
PMCID: PMC361544  PMID: 1981686

Abstract

Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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