Fig. 3.

Impact of Cyp1b1 deletion on estrogen metabolism. (A) Comparison of estrogens and EM levels within the lungs of 129SvJ Cyp1b1-WT and Cyp1b1-KO mice as determined by LC-MS². Asterisks indicate the metabolites levels that differ significantly in WT and KO mice. (B and C) Expression of Cyp1a1 and Comt in the lungs of Cyp1b1-WT and Cyp1b1-KO mice. The transcript level of each gene was determined by quantitative RT–PCR and normalized against that of the housekeeping gene Hprt. The fold difference was calculated using the Δ ΔCt method. The levels for WT female mice have been set arbitrarily to 1. Values represent the mean ± SEM (n = 5 females and 4 males). *P ≤ 0.05, **P ≤ 0.01.