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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: Biochemistry. 2012 Dec 4;51(50):10075–10086. doi: 10.1021/bi3009104

Figure 6.

Figure 6

Concentration dependence of the observed rate constants of K48-linked IQF diubiquitin binding to USP2. In A and B the rate constants kobs, 1 and kobs, 2 determined by fitting the fluorescence data reporting the K48-linked IQF diubiquitin binding to USP2 (see figure 5) were plotted against the concentration of USP2. Individual microscopic binding constants were derived from linear regression analysis of the plot of kobs, 1 (A) and nonlinear curve fitting to a hyperbola of the plot of kobs, 2 (B).