Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1991 Dec;11(12):6257–6267. doi: 10.1128/mcb.11.12.6257

Transfer of nucleosomes from parental to replicated chromatin.

T Krude 1, R Knippers 1
PMCID: PMC361814  PMID: 1658628

Abstract

Simian virus 40 (SV40) minichromosomes were used as the substrate for in vitro replication. Protein-free SV40 DNA or plasmids, carrying the SV40 origin of replication, served as controls. Replicated minichromosomal DNA possessed constrained negative superhelicity indicative of the presence of nucleosomes. The topological state of replicated minichromosomal DNA was precisely determined by two-dimensional gel electrophoresis. We show that most or all nucleosomes, present on the replicated minichromosomal DNA, were derived from the parental minichromosome substrate. The mode and the rate of nucleosome transfer from parental to minichromosomal daughter DNA were not influenced by high concentrations of competing replicating and nonreplicating protein-free DNA, indicating that nucleosomes remain associated with DNA during the replication process. The data also show that parental nucleosomes were segregated to the replicated daughter DNA strands in a dispersive manner.

Full text

PDF
6257

Images in this article

6259Image
on p.6259
6261Image
on p.6261
6262Image
on p.6262
6262Image
on p.6262
6263Image
on p.6263
6264Image
on p.6264
6264Image
on p.6264
6265Image
on p.6265
6265Image
on p.6265

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bina-Stein M., Simpson R. T. Specific folding and contraction of DNA by histones H3 and H4. Cell. 1977 Jul;11(3):609–618. doi: 10.1016/0092-8674(77)90078-2. [DOI] [PubMed] [Google Scholar]
  2. Bonne-Andrea C., Wong M. L., Alberts B. M. In vitro replication through nucleosomes without histone displacement. Nature. 1990 Feb 22;343(6260):719–726. doi: 10.1038/343719a0. [DOI] [PubMed] [Google Scholar]
  3. Camerini-Otero R. D., Felsenfeld G. Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernel. Nucleic Acids Res. 1977;4(5):1159–1181. doi: 10.1093/nar/4.5.1159-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Challberg M. D., Kelly T. J. Animal virus DNA replication. Annu Rev Biochem. 1989;58:671–717. doi: 10.1146/annurev.bi.58.070189.003323. [DOI] [PubMed] [Google Scholar]
  5. Cusick M. E., DePamphilis M. L., Wassarman P. M. Dispersive segregation of nucleosomes during replication of simian virus 40 chromosomes. J Mol Biol. 1984 Sep 15;178(2):249–271. doi: 10.1016/0022-2836(84)90143-8. [DOI] [PubMed] [Google Scholar]
  6. Decker R. S., Yamaguchi M., Possenti R., Bradley M. K., DePamphilis M. L. In vitro initiation of DNA replication in simian virus 40 chromosomes. J Biol Chem. 1987 Aug 5;262(22):10863–10872. [PubMed] [Google Scholar]
  7. Fotedar R., Roberts J. M. Multistep pathway for replication-dependent nucleosome assembly. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6459–6463. doi: 10.1073/pnas.86.17.6459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gruss C., Gutierrez C., Burhans W. C., DePamphilis M. L., Koller T., Sogo J. M. Nucleosome assembly in mammalian cell extracts before and after DNA replication. EMBO J. 1990 Sep;9(9):2911–2922. doi: 10.1002/j.1460-2075.1990.tb07482.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Ishimi Y., Hirosumi J., Sato W., Sugasawa K., Yokota S., Hanaoka F., Yamada M. Purification and initial characterization of a protein which facilitates assembly of nucleosome-like structure from mammalian cells. Eur J Biochem. 1984 Aug 1;142(3):431–439. doi: 10.1111/j.1432-1033.1984.tb08305.x. [DOI] [PubMed] [Google Scholar]
  11. Jackson V. In vivo studies on the dynamics of histone-DNA interaction: evidence for nucleosome dissolution during replication and transcription and a low level of dissolution independent of both. Biochemistry. 1990 Jan 23;29(3):719–731. doi: 10.1021/bi00455a019. [DOI] [PubMed] [Google Scholar]
  12. Keller W. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876–4880. doi: 10.1073/pnas.72.12.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klausing K., Scheffner M., Scheidtmann K. H., Stahl H., Knippers R. DNA binding properties and replication activity of the T antigen related D2 phosphoprotein. Biochemistry. 1989 Mar 7;28(5):2238–2244. doi: 10.1021/bi00431a040. [DOI] [PubMed] [Google Scholar]
  14. Klempnauer K. H., Fanning E., Otto B., Knippers R. Maturation of newly replicated chromatin of simian virus 40 and its host cell. J Mol Biol. 1980 Feb 5;136(4):359–374. doi: 10.1016/0022-2836(80)90395-2. [DOI] [PubMed] [Google Scholar]
  15. Lee M. S., Garrard W. T. Transcription-induced nucleosome 'splitting': an underlying structure for DNase I sensitive chromatin. EMBO J. 1991 Mar;10(3):607–615. doi: 10.1002/j.1460-2075.1991.tb07988.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Li J. J., Kelly T. J. Simian virus 40 DNA replication in vitro. Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973–6977. doi: 10.1073/pnas.81.22.6973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Müller U., Zentgraf H., Eicken I., Keller W. Higher order structure of simian virus 40 chromatin. Science. 1978 Aug 4;201(4354):406–415. doi: 10.1126/science.208155. [DOI] [PubMed] [Google Scholar]
  18. Peck L. J., Wang J. C. Energetics of B-to-Z transition in DNA. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6206–6210. doi: 10.1073/pnas.80.20.6206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simanis V., Lane D. P. An immunoaffinity purification procedure for SV40 large T antigen. Virology. 1985 Jul 15;144(1):88–100. doi: 10.1016/0042-6822(85)90308-3. [DOI] [PubMed] [Google Scholar]
  20. Smith S., Stillman B. Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro. Cell. 1989 Jul 14;58(1):15–25. doi: 10.1016/0092-8674(89)90398-x. [DOI] [PubMed] [Google Scholar]
  21. Smith S., Stillman B. Stepwise assembly of chromatin during DNA replication in vitro. EMBO J. 1991 Apr;10(4):971–980. doi: 10.1002/j.1460-2075.1991.tb08031.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sogo J. M., Stahl H., Koller T., Knippers R. Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures. J Mol Biol. 1986 May 5;189(1):189–204. doi: 10.1016/0022-2836(86)90390-6. [DOI] [PubMed] [Google Scholar]
  23. Stahl H., Dröge P., Zentgraf H., Knippers R. A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system. J Virol. 1985 May;54(2):473–482. doi: 10.1128/jvi.54.2.473-482.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Stillman B. Chromatin assembly during SV40 DNA replication in vitro. Cell. 1986 May 23;45(4):555–565. doi: 10.1016/0092-8674(86)90287-4. [DOI] [PubMed] [Google Scholar]
  25. Stillman B. Initiation of eukaryotic DNA replication in vitro. Annu Rev Cell Biol. 1989;5:197–245. doi: 10.1146/annurev.cb.05.110189.001213. [DOI] [PubMed] [Google Scholar]
  26. Su R. T., DePamphilis M. L. Simian virus 40 DNA replication in isolated replicating viral chromosomes. J Virol. 1978 Oct;28(1):53–65. doi: 10.1128/jvi.28.1.53-65.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sugasawa K., Murakami Y., Miyamoto N., Hanaoka F., Ui M. Assembly of nascent DNA into nucleosome structures in simian virus 40 chromosomes by HeLa cell extract. J Virol. 1990 Oct;64(10):4820–4829. doi: 10.1128/jvi.64.10.4820-4829.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Weintraub H., Worcel A., Alberts B. A model for chromatin based upon two symmetrically paired half-nucleosomes. Cell. 1976 Nov;9(3):409–417. doi: 10.1016/0092-8674(76)90085-4. [DOI] [PubMed] [Google Scholar]
  29. Wiekowski M., Dröge P., Stahl H. Monoclonal antibodies as probes for a function of large T antigen during the elongation process of simian virus 40 DNA replication. J Virol. 1987 Feb;61(2):411–418. doi: 10.1128/jvi.61.2.411-418.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES