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. 1991 Jun;2(6):467–477. doi: 10.1091/mbc.2.6.467

Localization of transforming growth factor-beta 1 in mitochondria of murine heart and liver.

U I Heine 1, J K Burmester 1, K C Flanders 1, D Danielpour 1, E F Munoz 1, A B Roberts 1, M B Sporn 1
PMCID: PMC361831  PMID: 1883875

Abstract

Using both electron microscopic immunohistochemistry and cell fractionation techniques, we show that transforming growth factor-beta 1 (TGF-beta 1) is found in mitochondria of rat and mouse cardiac myocytes and rat hepatocytes. Four different polyclonal antibodies, raised against various epitopes encompassing the mature portion of the TGF-beta 1 molecule as well as the pro-region of its precursor, were used for the electron microscopy studies. The localization of TGF-beta 1 in mitochondria was confirmed by detection of the native peptide in mitochondria isolated from rat heart and liver; the majority of native TGF-beta 1 found in liver homogenates was recovered in highly pure mitochondrial fractions. The functional role of TGF-beta in the mitochondrion is unknown at present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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