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. 2001 Aug 24;25(6):343–348. doi: 10.1007/s002640100293

The cytotoxic effect of methotrexate loaded bone cement on osteosarcoma cell lines

H Kim 1, Y Park 1, J Oh 1, K Yoo 1, S Lee 1
PMCID: PMC3620791  PMID: 11820438

Abstract.

We mixed various amounts of methotrexate with bone cement and measured the absorbance daily for 4 weeks. The cytotoxic effects on SaOS2 and MG63 osteosarcoma cells were examined by the MTT assay, and analysed according to the methotrexate concentration and the elapsed time. The amount of eluted methotrexate was greatest during the first day, and then decreased rapidly reaching a plateau in the third week. The number of viable tumour cells decreased significantly after 72 h, and they were hardly seen after 1 week.

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