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. 1989 Jan;9(1):329–331. doi: 10.1128/mcb.9.1.329

A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

M Winey 1, I Edelman 1, M R Culbertson 1
PMCID: PMC362177  PMID: 2648132

Abstract

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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