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. 1990 Apr;10(4):1779–1783. doi: 10.1128/mcb.10.4.1779

Diabetes induced in male transgenic mice by expression of human H-ras oncoprotein in pancreatic beta cells.

S Efrat 1, N Fleischer 1, D Hanahan 1
PMCID: PMC362285  PMID: 2181284

Abstract

Transgenic mice expressing an insulin-promoted H-ras hybrid gene in pancreatic beta cells developed beta-cell degeneration and diabetes. The disease was manifested in male mice by hyperglycemia, glycosuria, and reduced plasma insulin levels, which appeared around 5 months of age and led to premature death. Histological analyses revealed large holes within the islets of Langerhans and a reduced number of beta cells. The destruction of the islets was not associated with an obvious inflammatory activity. Ultrastructural analysis showed extensive engorgement in the endoplasmic reticulum of the residual beta cells from diabetic males. The females carrying the insulin-promoted ras gene did not manifest any of the physiological abnormalities observed in males and showed only minor histological and ultrastructural changes, even at much greater ages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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