Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1990 Apr;10(4):1815–1817. doi: 10.1128/mcb.10.4.1815

brlA requires both zinc fingers to induce development.

T H Adams 1, H Deising 1, W E Timberlake 1
PMCID: PMC362292  PMID: 2108321

Abstract

Expression of the Aspergillus nidulans brlA gene induces a developmental pathway leading to the production of asexual spores. We have introduced mutations into brlA that are expected to disrupt either or both Cys2-His2 Zn(II) coordination sites postulated to exist in the BrlA polypeptide. The resultant brlA alleles fail to induce either the asexual reproductive pathway or the expression of development-specific genes. These data support the hypothesis that brlA encodes a nucleic acid-binding protein whose activity requires each of two zinc fingers.

Full text

PDF
1815

Images in this article

1816Image
on p.1816
1816Image
on p.1816

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. H., Boylan M. T., Timberlake W. E. brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell. 1988 Jul 29;54(3):353–362. doi: 10.1016/0092-8674(88)90198-5. [DOI] [PubMed] [Google Scholar]
  2. Boylan M. T., Mirabito P. M., Willett C. E., Zimmerman C. R., Timberlake W. E. Isolation and physical characterization of three essential conidiation genes from Aspergillus nidulans. Mol Cell Biol. 1987 Sep;7(9):3113–3118. doi: 10.1128/mcb.7.9.3113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clutterbuck A. J. A mutational analysis of conidial development in Aspergillus nidulans. Genetics. 1969 Oct;63(2):317–327. doi: 10.1093/genetics/63.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evans R. M., Hollenberg S. M. Zinc fingers: gilt by association. Cell. 1988 Jan 15;52(1):1–3. doi: 10.1016/0092-8674(88)90522-3. [DOI] [PubMed] [Google Scholar]
  5. Gwynne D. I., Buxton F. P., Sibley S., Davies R. W., Lockington R. A., Scazzocchio C., Sealy-Lewis H. M. Comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in Aspergillus nidulans. Gene. 1987;51(2-3):205–216. doi: 10.1016/0378-1119(87)90309-x. [DOI] [PubMed] [Google Scholar]
  6. Hamer J. E., Timberlake W. E. Functional organization of the Aspergillus nidulans trpC promoter. Mol Cell Biol. 1987 Jul;7(7):2352–2359. doi: 10.1128/mcb.7.7.2352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kunkel T. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A. 1985 Jan;82(2):488–492. doi: 10.1073/pnas.82.2.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miller J., McLachlan A. D., Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985 Jun;4(6):1609–1614. doi: 10.1002/j.1460-2075.1985.tb03825.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mirabito P. M., Adams T. H., Timberlake W. E. Interactions of three sequentially expressed genes control temporal and spatial specificity in Aspergillus development. Cell. 1989 Jun 2;57(5):859–868. doi: 10.1016/0092-8674(89)90800-3. [DOI] [PubMed] [Google Scholar]
  10. Oliver P. T. Conidiophore and spore development in Aspergillus nidulans. J Gen Microbiol. 1972 Nov;73(1):45–54. doi: 10.1099/00221287-73-1-45. [DOI] [PubMed] [Google Scholar]
  11. Redemann N., Gaul U., Jäckle H. Disruption of a putative Cys-zinc interaction eliminates the biological activity of the Krüppel finger protein. Nature. 1988 Mar 3;332(6159):90–92. doi: 10.1038/332090a0. [DOI] [PubMed] [Google Scholar]
  12. Timberlake W. E., Marshall M. A. Genetic regulation of development in Aspergillus nidulans. Trends Genet. 1988 Jun;4(6):162–169. doi: 10.1016/0168-9525(88)90022-4. [DOI] [PubMed] [Google Scholar]
  13. Yelton M. M., Hamer J. E., Timberlake W. E. Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1470–1474. doi: 10.1073/pnas.81.5.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES