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. 1989 Jun;9(6):2734–2737. doi: 10.1128/mcb.9.6.2734

A transfected L-myc gene can substitute for c-myc in blocking murine erythroleukemia differentiation.

M J Birrer 1, L Raveh 1, H Dosaka 1, S Segal 1
PMCID: PMC362347  PMID: 2668739

Abstract

We investigated the ability of the proto-oncogene L-myc to substitute for c-myc in blocking murine erythroleukemia differentiation. Murine erythroleukemia cells (line C19) were transfected with recombinant plasmids containing genomic and cDNA fragments of the L-myc gene driven by a Moloney murine leukemia virus long terminal repeat. Clones expressing constitutive high levels of L-myc failed to differentiate in response to the chemical inducer N,N'-hexamethylene bisacetamide (HMBA). The block to differentiation correlated with the level of L-myc expression. Furthermore, transfected clones grown in the presence of inducer for an extended period of time showed an increased level of L-myc expression. These results suggest that functional domains of the c-myc gene involved in differentiation are located in the discrete regions of homology between the c- and L-myc genes.

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Selected References

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