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Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1989 Jun;9(6):2761–2764. doi: 10.1128/mcb.9.6.2761

Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers.

D Shippen-Lentz 1, E H Blackburn 1
PMCID: PMC362353  PMID: 2474761

Abstract

A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3'-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.

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Selected References

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