Skip to main content
NCBI home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1989 Sep;9(9):4105–4108. doi: 10.1128/mcb.9.9.4105

Calcium phosphate-mediated transfection alters metallothionein gene expression in response to Cd2+ and Zn2+.

R Foster 1, P E Olsson 1, L Gedamu 1
PMCID: PMC362482  PMID: 2506442

Abstract

The level of expression of a transfected metallothionein (MT)-IGcat fusion gene in response to cadmium differed from that of the endogenous MT-IG gene. Atomic absorption analysis indicated that the total cellular content of cadmium and zinc increased upon calcium phosphate-mediated transfection. Thus, changes in the influx/efflux of metals may regulate the level of MT gene expression.

Full text

PDF
4105

Images in this article

4105Image
on p.4105
4106Image
on p.4106

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Foster R., Jahroudi N., Varshney U., Gedamu L. Structure and expression of the human metallothionein-IG gene. Differential promoter activity of two linked metallothionein-I genes in response to heavy metals. J Biol Chem. 1988 Aug 15;263(23):11528–11535. [PubMed] [Google Scholar]
  3. Gorman C. M., Moffat L. F., Howard B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982 Sep;2(9):1044–1051. doi: 10.1128/mcb.2.9.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hamer D. H. Metallothionein. Annu Rev Biochem. 1986;55:913–951. doi: 10.1146/annurev.bi.55.070186.004405. [DOI] [PubMed] [Google Scholar]
  5. Heguy A., West A., Richards R. I., Karin M. Structure and tissue-specific expression of the human metallothionein IB gene. Mol Cell Biol. 1986 Jun;6(6):2149–2157. doi: 10.1128/mcb.6.6.2149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Karin M., Richards R. I. Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature. 1982 Oct 28;299(5886):797–802. doi: 10.1038/299797a0. [DOI] [PubMed] [Google Scholar]
  7. Knowles B. B., Howe C. C., Aden D. P. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science. 1980 Jul 25;209(4455):497–499. doi: 10.1126/science.6248960. [DOI] [PubMed] [Google Scholar]
  8. Kägi J. H., Vasák M., Lerch K., Gilg D. E., Hunziker P., Bernhard W. R., Good M. Structure of mammalian metallothionein. Environ Health Perspect. 1984 Mar;54:93–103. doi: 10.1289/ehp.54-1568188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pine R., Levy D. E., Reich N., Darnell J. E., Jr Transcriptional stimulation by CaPO4-DNA precipitates. Nucleic Acids Res. 1988 Feb 25;16(4):1371–1378. doi: 10.1093/nar/16.4.1371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pressman B. C. Biological applications of ionophores. Annu Rev Biochem. 1976;45:501–530. doi: 10.1146/annurev.bi.45.070176.002441. [DOI] [PubMed] [Google Scholar]
  11. Richards R. I., Heguy A., Karin M. Structural and functional analysis of the human metallothionein-IA gene: differential induction by metal ions and glucocorticoids. Cell. 1984 May;37(1):263–272. doi: 10.1016/0092-8674(84)90322-2. [DOI] [PubMed] [Google Scholar]
  12. Sadhu C., Gedamu L. Regulation of human metallothionein (MT) genes. Differential expression of MTI-F, MTI-G, and MTII-A genes in the hepatoblastoma cell line (HepG2). J Biol Chem. 1988 Feb 25;263(6):2679–2684. [PubMed] [Google Scholar]
  13. Schmidt C. J., Hamer D. H. Cell specificity and an effect of ras on human metallothionein gene expression. Proc Natl Acad Sci U S A. 1986 May;83(10):3346–3350. doi: 10.1073/pnas.83.10.3346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schmidt C. J., Jubier M. F., Hamer D. H. Structure and expression of two human metallothionein-I isoform genes and a related pseudogene. J Biol Chem. 1985 Jun 25;260(12):7731–7737. [PubMed] [Google Scholar]
  15. Seguin C., Hamer D. H. Regulation in vitro of metallothionein gene binding factors. Science. 1987 Mar 13;235(4794):1383–1387. doi: 10.1126/science.3103216. [DOI] [PubMed] [Google Scholar]
  16. Varshney U., Jahroudi N., Foster R., Gedamu L. Structure, organization, and regulation of human metallothionein IF gene: differential and cell-type-specific expression in response to heavy metals and glucocorticoids. Mol Cell Biol. 1986 Jan;6(1):26–37. doi: 10.1128/mcb.6.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Westin G., Schaffner W. A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene. EMBO J. 1988 Dec 1;7(12):3763–3770. doi: 10.1002/j.1460-2075.1988.tb03260.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES