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. 1989 Sep;9(9):4113–4117. doi: 10.1128/mcb.9.9.4113

Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.

G J Sorger 1, D Brown 1, M Farzannejad 1, A Guerra 1, M Jonathan 1, S Knight 1, R Sharda 1
PMCID: PMC362484  PMID: 2528690

Abstract

Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.

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Selected References

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