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. 1989 Oct;9(10):4576–4580. doi: 10.1128/mcb.9.10.4576

Evidence for glycosyl-phosphatidylinositol anchoring of Toxoplasma gondii major surface antigens.

S Tomavo 1, R T Schwarz 1, J F Dubremetz 1
PMCID: PMC362548  PMID: 2531282

Abstract

The four major surface antigens of Toxoplasma gondii tachyzoites (P43, P35, P30, and P22) were made water soluble by phosphatidylinositol-specific phospholipase C (PI-PLC). These antigens were biosynthetically labeled with 3H-fatty acids, [3H]ethanolamine, and [3H]carbohydrates. Treatment of 3H-fatty-acid-labeled parasite lysates with PI-PLC removed the radioactive label from these antigens. A cross-reacting determinant was exposed on these antigens after PI-PLC treatment.

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Selected References

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