Skip to main content
PMC home page
Molecular and Cellular Biology logoLink to Molecular and Cellular Biology
. 1989 Feb;9(2):865–868. doi: 10.1128/mcb.9.2.865

Detection of a Myc-associated protein by chemical cross-linking.

D A Gillespie 1, R N Eisenman 1
PMCID: PMC362670  PMID: 2651907

Abstract

A single nuclear protein (Myc-associated protein) can be specifically cross-linked to avian Myc proteins by treatment of nuclei or cells with the reversible cross-linker dimethyl 3,3'-dithiobis-propionimidate. Myc-associated protein has a molecular weight of approximately 500,000, is not detectably phosphorylated and, in contrast to Myc, has a long apparent half-life of greater than 3 h.

Full text

PDF
865

Images in this article

866Image
on p.866
866Image
on p.866
867Image
on p.867
867Image
on p.867

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cole M. D. The myc oncogene: its role in transformation and differentiation. Annu Rev Genet. 1986;20:361–384. doi: 10.1146/annurev.ge.20.120186.002045. [DOI] [PubMed] [Google Scholar]
  2. Cory S. Activation of cellular oncogenes in hemopoietic cells by chromosome translocation. Adv Cancer Res. 1986;47:189–234. doi: 10.1016/s0065-230x(08)60200-6. [DOI] [PubMed] [Google Scholar]
  3. Eisenman R. N., Tachibana C. Y., Abrams H. D., Hann S. R. V-myc- and c-myc-encoded proteins are associated with the nuclear matrix. Mol Cell Biol. 1985 Jan;5(1):114–126. doi: 10.1128/mcb.5.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenman R. N., Thompson C. B. Oncogenes with potential nuclear function: myc, myb and fos. Cancer Surv. 1986;5(2):309–327. [PubMed] [Google Scholar]
  5. Evan G. I., Hancock D. C. Studies on the interaction of the human c-myc protein with cell nuclei: p62c-myc as a member of a discrete subset of nuclear proteins. Cell. 1985 Nov;43(1):253–261. doi: 10.1016/0092-8674(85)90030-3. [DOI] [PubMed] [Google Scholar]
  6. Finlay C. A., Hinds P. W., Tan T. H., Eliyahu D., Oren M., Levine A. J. Activating mutations for transformation by p53 produce a gene product that forms an hsc70-p53 complex with an altered half-life. Mol Cell Biol. 1988 Feb;8(2):531–539. doi: 10.1128/mcb.8.2.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hann S. R., Abrams H. D., Rohrschneider L. R., Eisenman R. N. Proteins encoded by v-myc and c-myc oncogenes: identification and localization in acute leukemia virus transformants and bursal lymphoma cell lines. Cell. 1983 Oct;34(3):789–798. doi: 10.1016/0092-8674(83)90535-4. [DOI] [PubMed] [Google Scholar]
  8. Hann S. R., Eisenman R. N. Proteins encoded by the human c-myc oncogene: differential expression in neoplastic cells. Mol Cell Biol. 1984 Nov;4(11):2486–2497. doi: 10.1128/mcb.4.11.2486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaddurah-Daouk R., Greene J. M., Baldwin A. S., Jr, Kingston R. E. Activation and repression of mammalian gene expression by the c-myc protein. Genes Dev. 1987 Jun;1(4):347–357. doi: 10.1101/gad.1.4.347. [DOI] [PubMed] [Google Scholar]
  10. King M. W., Roberts J. M., Eisenman R. N. Expression of the c-myc proto-oncogene during development of Xenopus laevis. Mol Cell Biol. 1986 Dec;6(12):4499–4508. doi: 10.1128/mcb.6.12.4499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lane D. P. p53 in Paris, an oncogene comes of age. Oncogene. 1987;1(3):241–242. [PubMed] [Google Scholar]
  12. Lüscher B., Eisenman R. N. c-myc and c-myb protein degradation: effect of metabolic inhibitors and heat shock. Mol Cell Biol. 1988 Jun;8(6):2504–2512. doi: 10.1128/mcb.8.6.2504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spector D. L., Watt R. A., Sullivan N. F. The v- and c-myc oncogene proteins colocalize in situ with small nuclear ribonucleoprotein particles. Oncogene. 1987 Mar;1(1):5–12. [PubMed] [Google Scholar]
  14. Studzinski G. P., Brelvi Z. S., Feldman S. C., Watt R. A. Participation of c-myc protein in DNA synthesis of human cells. Science. 1986 Oct 24;234(4775):467–470. doi: 10.1126/science.3532322. [DOI] [PubMed] [Google Scholar]
  15. Varmus H. E. Oncogenes and transcriptional control. Science. 1987 Dec 4;238(4832):1337–1339. doi: 10.1126/science.2825348. [DOI] [PubMed] [Google Scholar]
  16. Wang K., Richards F. M. An approach to nearest neighbor analysis of membrane proteins. Application to the human erythrocyte membrane of a method employing cleavable cross-linkages. J Biol Chem. 1974 Dec 25;249(24):8005–8018. [PubMed] [Google Scholar]
  17. Watt R. A., Shatzman A. R., Rosenberg M. Expression and characterization of the human c-myc DNA-binding protein. Mol Cell Biol. 1985 Mar;5(3):448–456. doi: 10.1128/mcb.5.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

RESOURCES