Abstract
We isolated a cDNA for p20K, a secreted protein preferentially synthesized in nonproliferating cells. p20K mRNA and protein levels declined rapidly following treatment with various mitogens. DNA sequence analysis of the p20K cDNA predicted a novel protein distantly related to alpha 2 mu-globulin and plasma retinol-binding protein.
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- Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
- Balk S. D. Active proliferation of Rous sarcoma virus-infected, but not normal, chicken heart mesenchymal cells in culture medium of physiological composition. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6606–6610. doi: 10.1073/pnas.77.11.6606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balk S. D., Gunther H. S., Morisi A. Morphological transformation, autonomous proliferation and colony formation by chicken heart mesenchymal cells infected with avian sarcoma, erythroblastosis and myelocytomatosis viruses. Life Sci. 1984 Sep 10;35(11):1157–1171. doi: 10.1016/0024-3205(84)90186-3. [DOI] [PubMed] [Google Scholar]
- Balk S. D., Morisi A., Gunther H. S., Svoboda M. F., Van Wyk J. J., Nissley S. P., Scanes C. G. Somatomedins (insulin-like growth factors), but not growth hormone, are mitogenic for chicken heart mesenchymal cells and act synergistically with epidermal growth factor and brain fibroblast growth factor. Life Sci. 1984 Jul 23;35(4):335–346. doi: 10.1016/0024-3205(84)90643-x. [DOI] [PubMed] [Google Scholar]
- Balk S. D., Shiu R. P., LaFleur M. M., Young L. L. Epidermal growth factor and insulin cause normal chicken heart mesenchymal cells to proliferate like their Rous sarcoma virus-infected counterparts. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1154–1157. doi: 10.1073/pnas.79.4.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bedard P. A., Alcorta D., Simmons D. L., Luk K. C., Erikson R. L. Constitutive expression of a gene encoding a polypeptide homologous to biologically active human platelet protein in Rous sarcoma virus-transformed fibroblasts. Proc Natl Acad Sci U S A. 1987 Oct;84(19):6715–6719. doi: 10.1073/pnas.84.19.6715. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks D. E. The major androgen-regulated secretory proteins of the rat epididymis bear sequence homology with members of the alpha 2u-globulin superfamily. Biochem Int. 1987 Feb;14(2):235–240. [PubMed] [Google Scholar]
- Bédard P. A., Balk S. D., Gunther H. S., Morisi A., Erikson R. L. Repression of quiescence-specific polypeptides in chicken heart mesenchymal cells transformed by Rous sarcoma virus. Mol Cell Biol. 1987 Apr;7(4):1450–1458. doi: 10.1128/mcb.7.4.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chou P. Y., Fasman G. D. Beta-turns in proteins. J Mol Biol. 1977 Sep 15;115(2):135–175. doi: 10.1016/0022-2836(77)90094-8. [DOI] [PubMed] [Google Scholar]
- Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
- Cochran B. H., Reffel A. C., Stiles C. D. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell. 1983 Jul;33(3):939–947. doi: 10.1016/0092-8674(83)90037-5. [DOI] [PubMed] [Google Scholar]
- Conti A., Godovac-Zimmermann J., Liberatori J., Braunitzer G. The primary structure of monomeric beta-lactoglobulin I from horse colostrum (Equus caballus, Perissodactyla). Hoppe Seylers Z Physiol Chem. 1984 Dec;365(12):1393–1401. doi: 10.1515/bchm2.1984.365.2.1393. [DOI] [PubMed] [Google Scholar]
- Fagan J. B., Sobel M. E., Yamada K. M., de Crombrugghe B., Pastan I. Effects of transformation on fibronectin gene expression using cloned fibronectin cDNA. J Biol Chem. 1981 Jan 10;256(1):520–525. [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Greenberg M. E., Ziff E. B. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984 Oct 4;311(5985):433–438. doi: 10.1038/311433a0. [DOI] [PubMed] [Google Scholar]
- Lau L. F., Nathans D. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells. EMBO J. 1985 Dec 1;4(12):3145–3151. doi: 10.1002/j.1460-2075.1985.tb04057.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee K. H., Wells R. G., Reed R. R. Isolation of an olfactory cDNA: similarity to retinol-binding protein suggests a role in olfaction. Science. 1987 Feb 27;235(4792):1053–1056. doi: 10.1126/science.3493528. [DOI] [PubMed] [Google Scholar]
- Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
- Rask L., Anundi H., Böhme J., Eriksson U., Ronne H., Sege K., Peterson P. A. Structural and functional studies of vitamin A-binding proteins. Ann N Y Acad Sci. 1981 Feb 27;359:79–90. doi: 10.1111/j.1749-6632.1981.tb12739.x. [DOI] [PubMed] [Google Scholar]
- Russel M., Kidd S., Kelley M. R. An improved filamentous helper phage for generating single-stranded plasmid DNA. Gene. 1986;45(3):333–338. doi: 10.1016/0378-1119(86)90032-6. [DOI] [PubMed] [Google Scholar]
- Sigel M. B., Sinha Y. N., VanderLaan W. P. Production of antibodies by inoculation into lymph nodes. Methods Enzymol. 1983;93:3–12. doi: 10.1016/s0076-6879(83)93031-8. [DOI] [PubMed] [Google Scholar]
- Unterman R. D., Lynch K. R., Nakhasi H. L., Dolan K. P., Hamilton J. W., Cohn D. V., Feigelson P. Cloning and sequence of several alpha 2u-globulin cDNAs. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3478–3482. doi: 10.1073/pnas.78.6.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. doi: 10.1111/j.1432-1033.1983.tb07424.x. [DOI] [PubMed] [Google Scholar]