Abstract
An indirect immunoferritin (IIF) technique is described for recognizing the elementary bodies (EB) of Chlamydia trachomatis in unsectioned preparations. Both the EB of a genital strain of C. trachomatis grown in irradiated McCoy cells and EB in clinical specimens obtained from patients attending a venereal disease clinic were identified by the IIF test in the electron microscope. Cell culture-grown EB were detected by ferritin staining for up to 4 weeks after the organisms had lost their infectivity for tissue cultures. The IIF test was of comparable sensitivity to isolation methods in detecting chlamydiae in clinical specimens. Other possible applications of the IIF technique are discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAXANDALL J., PERLMANN P., AFZELIUS B. A. A two-layer technique for detecting surface antigens in the sea urchin egg with ferritin-conjugated antibody. J Cell Biol. 1962 Jul;14:144–151. doi: 10.1083/jcb.14.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
- Barenfanger J., MacDonald A. B. The role of immunoglobulin in the neutralization of trachoma infectivity. J Immunol. 1974 Nov;113(5):1607–1617. [PubMed] [Google Scholar]
- Dhir S. P., Boatman E. S. Location of polysaccharide on Chlamydia psittaci by silver-methenamine staining and electron microscopy. J Bacteriol. 1972 Jul;111(1):267–271. doi: 10.1128/jb.111.1.267-271.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moulder J. W. The relation of the psittacosis group (Chlamydiae) to bacteria and viruses. Annu Rev Microbiol. 1966;20:107–130. doi: 10.1146/annurev.mi.20.100166.000543. [DOI] [PubMed] [Google Scholar]
- Paver W. K., Caul E. O., Clarke S. K. Comparison of 22 nm virus from human faeces with animal parvoviruses. J Gen Virol. 1974 Mar;22(3):447–450. doi: 10.1099/0022-1317-22-3-447. [DOI] [PubMed] [Google Scholar]
- Richmond S. J., Caul E. O. Fluorescent antibody studies in chlamydial infections. J Clin Microbiol. 1975 Apr;1(4):345–352. doi: 10.1128/jcm.1.4.345-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Richmond S. J. The isolation of Chlamydia subgroup A (Chlamydia trachomatis) in irradiated McCoy cells. Med Lab Technol. 1974 Jan;31(1):7–9. [PubMed] [Google Scholar]
- SINGER S. J., MCLEAN J. D. FERRITIN-ANTIBODY CONJUGATES AS STAINS FOR ELECTRON MICROSCOPY. Lab Invest. 1963 Oct;12:1002–1008. [PubMed] [Google Scholar]
- SINGER S. J. Preparation of an electron-dense antibody conjugate. Nature. 1959 May 30;183(4674):1523–1524. doi: 10.1038/1831523a0. [DOI] [PubMed] [Google Scholar]
- Sompolinsky D., Richmond S. Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B. Appl Microbiol. 1974 Dec;28(6):912–914. doi: 10.1128/am.28.6.912-914.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sowa J., Collier L. H., Sowa S. A comparison of the iodine and fluorescent antibody methods for staining trachoma inclusions in the conjunctiva. J Hyg (Lond) 1971 Dec;69(4):693–708. doi: 10.1017/s0022172400021963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]