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. 1975 Oct;2(4):345–348. doi: 10.1128/jcm.2.4.345-348.1975

Germ tube and chlamydospore formation by Candida albicans on a new medium.

F Beheshti, A G Smith, G W Krause
PMCID: PMC362807  PMID: 1102561

Abstract

A new medium composed of "cream of rice" infusion, oxgall, Tween 80, and agar is described for the sequential development of germ tubes and chlamydospores by Candida albicans. The procedure used (Dalmau's technique) is an improvement over the fluid substrate procedures previously advocated for germ tube formation. That the same preparation is then used for chlamydospore production is of practical importance for the clinical mycology laboratory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDLEIGH H. S. RAPID IDENTIFICATION OF CANDIDA ALBICANS. Mycopathol Mycol Appl. 1964 Aug 15;23:81–84. doi: 10.1007/BF02049262. [DOI] [PubMed] [Google Scholar]
  2. Dolan C. T., Ihrke D. M. Further studies of the germ-tube test for Candida albicans identification. Am J Clin Pathol. 1971 Jun;55(6):733–734. doi: 10.1093/ajcp/55.6.733. [DOI] [PubMed] [Google Scholar]
  3. Fell J. W., Meyer S. A. Systematics of yeast species in the Candida parapsilosis group. Mycopathol Mycol Appl. 1967 Aug 14;32(3):177–193. doi: 10.1007/BF02049795. [DOI] [PubMed] [Google Scholar]
  4. Fischer J. B., Kane J. Production of chlamydospores by Candida albicans cultivated on dilute oxgall agar. Mycopathol Mycol Appl. 1968 Oct 14;35(3):223–232. doi: 10.1007/BF02050734. [DOI] [PubMed] [Google Scholar]
  5. HU F., LIVINGOOD C. S., JOHNSON P., POMERAT C. M. Tissue culture studies on human skin. V. Conjoint culture of Candida albicans, human skin, and embryonic chick heart; inhibitory effects of endomycin, candicidin A, and asterol on C albicans grown conjointly with human skin and embryonic chick heart. AMA Arch Derm Syphilol. 1954 Jul;70(1):1–15. doi: 10.1001/archderm.1954.01540190003001. [DOI] [PubMed] [Google Scholar]
  6. Hayes A. B. Chlamydospore production in Candida albicans. Mycopathol Mycol Appl. 1966 May 31;29(1):87–96. doi: 10.1007/BF02055062. [DOI] [PubMed] [Google Scholar]
  7. Joshi K. R., Bremner D. A., Gavin J. B., Herdson P. B., Parr D. N. The formation of germ tubes by Candida albicans in sheep serum and trypticase soya broth. Am J Clin Pathol. 1973 Dec;60(6):839–842. doi: 10.1093/ajcp/60.6.839. [DOI] [PubMed] [Google Scholar]
  8. Joshi K. R., Gavin J. B., Bremner D. A. The formation of germ tubes by Candida albicans in various peptone media. Sabouraudia. 1973 Nov;11(3):259–262. [PubMed] [Google Scholar]
  9. Kamaya T. Simple rapid identification of Candida albicans with emphasis on the differentiation between Candida albicans and Candida stellatoidea. Mycopathol Mycol Appl. 1968 Jul 12;35(2):105–112. doi: 10.1007/BF02049573. [DOI] [PubMed] [Google Scholar]
  10. Katsura Y., Uesaka I. Assessment of germ tube dispersion activity of serum from experimental candidiasis: a new procedure for serodiagnosis. Infect Immun. 1974 May;9(5):788–793. doi: 10.1128/iai.9.5.788-793.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LANDAU J. W., DABROWA N., NEWCOMER V. D., ROWE J. R. THE RELATIONSHIP OF SERUM TRANSFERRIN AND IRON TO THE RAPID FORMATION OF GERM TUBES BY CANDIDA ALBICANS. J Invest Dermatol. 1964 Dec;43:473–482. [PubMed] [Google Scholar]
  12. Mackenzie D. W. Morphogenesis of Candida albicans in vivo. Sabouraudia. 1964 Jun;3(3):225–232. [PubMed] [Google Scholar]
  13. Mackenzie D. W. Serum tube identification of Candida albicans. J Clin Pathol. 1962 Nov;15(6):563–565. doi: 10.1136/jcp.15.6.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McGinnis M. R., Padhye A. A., Ajello L. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic actinomycetes in sterile distilled water. Appl Microbiol. 1974 Aug;28(2):218–222. doi: 10.1128/am.28.2.218-222.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RAUBITSCHEK F. Taurocholate agar for chlamydospore production by Candida albicans. Mycopathol Mycol Appl. 1958 Sep 29;9(4):285–287. doi: 10.1007/BF02051532. [DOI] [PubMed] [Google Scholar]
  16. SMITH A. G., TAUBERT H. D., TOWNS C. M. Comparative media studies in the isolation of Candida albicans from pregnant women. Mycopathol Mycol Appl. 1962 Jul 30;17:269–280. doi: 10.1007/BF02279300. [DOI] [PubMed] [Google Scholar]
  17. Schaar G., Long I., Widra A. A combination rapid and standard method for identification of Candida albicans. Mycopathol Mycol Appl. 1974 Apr 30;52(3):203–207. doi: 10.1007/BF02198744. [DOI] [PubMed] [Google Scholar]
  18. TASCHDJIAN C. L., BURCHALL J. J., KOZINN P. J. Rapid identification of Candida albicans by filamentation on serum and serum substitutes. AMA J Dis Child. 1960 Feb;99:212–215. doi: 10.1001/archpedi.1960.02070030214011. [DOI] [PubMed] [Google Scholar]
  19. TAUBERT H. D., SMITH A. G. The clinical use of Taschdjian's medium in the diagnosis of vulvovaginal candidiasis. J Lab Clin Med. 1960 May;55:820–828. [PubMed] [Google Scholar]

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