Figure 2. lz and Hr39 are not required in reproductive tract neurons.
(A) lz expression (lzGal4 driving UAS-mCD8::GFP) in control female reproductive tract. Spermathecae (yellow arrowheads); parovaria (magenta arrowheads). Ov: Ovuduct; SR: Seminal receptacle; Ut: Uterus. Two sets of lz+ sensory neurons are illustrated at higher magnification in (A1 and A2). (B) lz expression in female reproductive tract expressing lzGal4>UAScycA (lz>cycARNAi). lz+ sensory neurons are not affected (B1 and B2). (C) Egg production is not affected by expressing Hr39-RNAi in ppk+ neurons of the reproductive tract. (D) Ectopic expression of mSP in fru+ reproductive tract neurons reduces virgin female copulation rate, even when neurons are mutant for Hr39. (E) Ectopic mSP in fru+ neurons is sufficient to induce egg laying in control virgin females but not in Hr39−/− females even in the presence of males. * indicates p<0.01 and NS indicates p>0.05.