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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1986 Apr;23(4):667–671. doi: 10.1128/jcm.23.4.667-671.1986

Serodiagnosis of La Crosse virus infections in humans by detection of immunoglobulin M class antibodies.

C H Calisher, C I Pretzman, D J Muth, M A Parsons, E D Peterson
PMCID: PMC362813  PMID: 3700625

Abstract

Sera from 92 humans with illnesses clinically compatible with those caused by California serogroup virus infections were tested for antibody to La Crosse (LAC) virus by using the immunoglobulin M (IgM) antibody-capture enzyme-linked immunosorbent assay (MAC ELISA), the IgG ELISA, and the hemagglutination inhibition (HI), complement fixation and serum dilution-plaque reduction neutralization tests. On the reported day of onset of illness in 18 individuals, 94% had IgM antibody, 50% had neutralization antibody, 33% had HI antibody, and 11% had IgG antibody. Neutralization, HI, and IgG antibody prevalence rates increased thereafter, whereas IgM antibody prevalence remained high (92% 2 or more weeks after the onset of illness). It was concluded that the MAC ELISA is a sensitive test for the presence of antibody to LAC virus. The sensitivity of the MAC ELISA and the rapidity with which it can be performed appear to provide a powerful tool for the clinically relevant serodiagnosis of LAC virus infections in humans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaty B. J., Jamnback T. L., Hildreth S. W., Brown K. L. Rapid diagnosis of La Crosse virus infections: evaluation of serologic and antigen detection techniques for the clinically relevant diagnosis of La Crosse encephalitis. Prog Clin Biol Res. 1983;123:293–302. [PubMed] [Google Scholar]
  2. Calisher C. H., Bailey R. E. Serodiagnosis of La Crosse virus infections in humans. J Clin Microbiol. 1981 Feb;13(2):344–350. doi: 10.1128/jcm.13.2.344-350.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Calisher C. H. Taxonomy, classification, and geographic distribution of California serogroup bunyaviruses. Prog Clin Biol Res. 1983;123:1–16. [PubMed] [Google Scholar]
  4. Calisher C. H., el-Kafrawi A. O., Al-Deen Mahmud M. I., Travassos da Rosa A. P., Bartz C. R., Brummer-Korvenkontio M., Haksohusodo S., Suharyono W. Complex-specific immunoglobulin M antibody patterns in humans infected with alphaviruses. J Clin Microbiol. 1986 Jan;23(1):155–159. doi: 10.1128/jcm.23.1.155-159.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chappell W. A., Halonen P. E., Toole R. F., Calisher C. H., Chester L. Preparation of La Crosse virus hemagglutinating antigen in BHK-21 suspension cell cultures. Appl Microbiol. 1969 Sep;18(3):433–437. doi: 10.1128/am.18.3.433-437.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Deering W. M. Neurologic aspects and treatment of La Crosse encephalitis. Prog Clin Biol Res. 1983;123:187–191. [PubMed] [Google Scholar]
  7. Deibel R., Srihongse S., Grayson M. A., Grimstad P. R., Mahdy M. S., Artsob H., Calisher C. H. Jamestown Canyon virus: the etiologic agent of an emerging human disease? Prog Clin Biol Res. 1983;123:313–325. [PubMed] [Google Scholar]
  8. Fauvel M., Artsob H., Calisher C. H., Davignon L., Chagnon A., Skvorc-Ranko R., Belloncik S. California group virus encephalitis in three children from Quebec: clinical and serologic findings. Can Med Assoc J. 1980 Jan 12;122(1):60-2, 64. [PMC free article] [PubMed] [Google Scholar]
  9. Gundersen C. B., Brown K. L. Clinical aspects of La Crosse encephalitis: preliminary report. Prog Clin Biol Res. 1983;123:169–177. [PubMed] [Google Scholar]
  10. Jamnback T. L., Beaty B. J., Hildreth S. W., Brown K. L., Gundersen C. B. Capture immunoglobulin M system for rapid diagnosis of La Crosse (California encephalitis) virus infections. J Clin Microbiol. 1982 Sep;16(3):577–580. doi: 10.1128/jcm.16.3.577-580.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klimas R. A., Thompson W. H., Calisher C. H., Clark G. G., Grimstad P. R., Bishop D. H. Genotypic varieties of La Crosse virus isolated from different geographic regions of the continental United States and evidence for a naturally occurring intertypic recombinant La Crosse virus. Am J Epidemiol. 1981 Jul;114(1):112–131. doi: 10.1093/oxfordjournals.aje.a113158. [DOI] [PubMed] [Google Scholar]
  12. Monath T. P. Arthropod-borne encephalitides in the Americas. Bull World Health Organ. 1979;57(4):513–533. [PMC free article] [PubMed] [Google Scholar]
  13. Monath T. P., Nystrom R. R., Bailey R. E., Calisher C. H., Muth D. J. Immunoglobulin M antibody capture enzyme-linked immunosorbent assay for diagnosis of St. Louis encephalitis. J Clin Microbiol. 1984 Oct;20(4):784–790. doi: 10.1128/jcm.20.4.784-790.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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