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. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986

Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining.

V C Salmon, R B Turner, M J Speranza, J C Overall Jr
PMCID: PMC362816  PMID: 3009538

Abstract

The effect of immunoperoxidase staining and centrifugation on the sensitivity and rapidity of herpes simplex virus detection in mink lung cell cultures was determined with 730 clinical specimens. In standard tube cultures, the use of immunoperoxidase staining resulted in detection of 31 (91%) of 34 positive cultures after overnight incubation, compared with 25 (74%) detected without the stain (P less than 0.05). The effect of centrifugation of specimens onto the monolayer followed by overnight incubation and immunoperoxidase staining was studied with 431 specimens. Of 107 positive specimens, 103 (96%) were detected by this method, compared with 91 (85%) detected in standard cell cultures observed for 5 days (P less than 0.02). Standard cell cultures that were examined after overnight incubation detected only 62 (58%) of the 107 positive specimens (P less than 0.001). Centrifugation of clinical specimens onto cell monolayers followed by overnight incubation and immunoperoxidase staining is more rapid and sensitive than are standard cell culture techniques for the laboratory diagnosis of herpes simplex virus infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Callihan D. R., Menegus M. A. Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures. J Clin Microbiol. 1984 Apr;19(4):563–565. doi: 10.1128/jcm.19.4.563-565.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fayram S. L., Aarnaes S., de la Maza L. M. Comparison of cultureset to a conventional tissue culture-fluorescent-antibody technique for isolation and identification of herpes simplex virus. J Clin Microbiol. 1983 Jul;18(1):215–216. doi: 10.1128/jcm.18.1.215-216.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hayden F. G., Sorensen A. S., Bateman J. A. Comparison of the Immulok cultureset kit and virus isolation for detection of herpes simplex virus in clinical specimens. J Clin Microbiol. 1983 Jul;18(1):222–224. doi: 10.1128/jcm.18.1.222-224.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson F. B., Leavitt R. W., Richards D. F. Comparison of the Scott Selecticult-HSV kit with conventional culture and direct immunoperoxidase staining for detection of herpes simplex virus in cultures of clinical specimens. J Clin Microbiol. 1985 Mar;21(3):438–441. doi: 10.1128/jcm.21.3.438-441.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mayo D. R., Brennan T., Egbertson S. H., Moore D. F. Rapid herpes simplex virus detection in clinical samples submitted to a state virology laboratory. J Clin Microbiol. 1985 May;21(5):768–771. doi: 10.1128/jcm.21.5.768-771.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller M. J., Howell C. L. Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure. J Clin Microbiol. 1983 Sep;18(3):550–553. doi: 10.1128/jcm.18.3.550-553.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moore D. F. Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus. J Clin Microbiol. 1984 Apr;19(4):548–549. doi: 10.1128/jcm.19.4.548-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nerurkar L. S., Jacob A. J., Madden D. L., Sever J. L. Detection of genital herpes simplex infections by a tissue culture-fluorescent-antibody technique with biotin-avidin. J Clin Microbiol. 1983 Jan;17(1):149–154. doi: 10.1128/jcm.17.1.149-154.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nerurkar L. S., Namba M., Brashears G., Jacob A. J., Lee Y. J., Sever J. L. Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay. J Clin Microbiol. 1984 Jul;20(1):109–114. doi: 10.1128/jcm.20.1.109-114.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nerurkar L. S., Namba M., Sever J. L. Comparison of standard tissue culture, tissue culture plus staining, and direct staining for detection of genital herpes simplex virus infection. J Clin Microbiol. 1984 May;19(5):631–633. doi: 10.1128/jcm.19.5.631-633.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phillips L. E., Magliolo R. A., Stehlik M. L., Whiteman P. A., Faro S., Rogers T. E. Retrospective evaluation of the isolation and identification of herpes simplex virus with Cultureset and human fibroblasts. J Clin Microbiol. 1985 Aug;22(2):255–258. doi: 10.1128/jcm.22.2.255-258.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Salmon V. C., Michaels B. K., Turner R. B. Comparison of cell culture with an immunoperoxidase kit for rapid diagnosis of herpes simplex virus infections. Diagn Microbiol Infect Dis. 1984 Sep;2(4):343–345. doi: 10.1016/0732-8893(84)90067-1. [DOI] [PubMed] [Google Scholar]
  14. Salmon V. C., Stanberry L. R., Overall J. C., Jr More rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in Vero or human fibroblast cells. Diagn Microbiol Infect Dis. 1984 Sep;2(4):317–324. doi: 10.1016/0732-8893(84)90063-4. [DOI] [PubMed] [Google Scholar]
  15. Tenser R. B. Ultracentrifugal inoculation of herpes simplex virus. Infect Immun. 1978 Jul;21(1):281–285. doi: 10.1128/iai.21.1.281-285.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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