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. 1986 Apr;23(4):752–754. doi: 10.1128/jcm.23.4.752-754.1986

Evaluation of an enzyme immunoassay for the diagnosis of chlamydial infections in urogenital specimens.

K H Tjiam, B Y van Heijst, A van Zuuren, J H Wagenvoort, T van Joost, E Stolz, M F Michel
PMCID: PMC362830  PMID: 3517062

Abstract

A total of 194 male urethral and 402 cervical specimens were obtained from patients at the venereal disease outpatient clinic of University Hospital, Rotterdam, The Netherlands, to evaluate the IDEIA test (Boots Celltech) for the detection of chlamydial infections. The prevalences of culture-positive males and females were 17.5 and 8.2%, respectively. The respective overall sensitivities and specificities found were 67.6 and 93.7% for the males and 63.6 and 93.8% for the females. The highest sensitivity (83.3%) was found in male patients with more than 20 leukocytes per field in the sediment of the first-voided urine (magnification, X250) and in women with more than 10 leukocytes per field in a cervical Gram stain (magnification, X800). However, in men without urethritis and in women with fewer than 10 leukocytes per field in the Gram stain, sensitivities of 44.4 and 40%, respectively, were found. Culture-positive, IDEIA-negative results were predominantly observed in samples with few inclusions in the culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brunham R. C., Paavonen J., Stevens C. E., Kiviat N., Kuo C. C., Critchlow C. W., Holmes K. K. Mucopurulent cervicitis--the ignored counterpart in women of urethritis in men. N Engl J Med. 1984 Jul 5;311(1):1–6. doi: 10.1056/NEJM198407053110101. [DOI] [PubMed] [Google Scholar]
  2. Caul E. O., Paul I. D. Monoclonal antibody based ELISA for detecting Chlamydia trachomatis. Lancet. 1985 Feb 2;1(8423):279–279. doi: 10.1016/s0140-6736(85)91056-6. [DOI] [PubMed] [Google Scholar]
  3. Jones M. F., Smith T. F., Houglum A. J., Herrmann J. E. Detection of Chlamydia trachomatis in genital specimens by the Chlamydiazyme test. J Clin Microbiol. 1984 Sep;20(3):465–467. doi: 10.1128/jcm.20.3.465-467.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kiviat N. B., Peterson M., Kinney-Thomas E., Tam M., Stamm W. E., Holmes K. K. Cytologic manifestations of cervical and vaginal infections. II. Confirmation of Chlamydia trachomatis infection by direct immunofluorescence using monoclonal antibodies. JAMA. 1985 Feb 15;253(7):997–1000. doi: 10.1001/jama.253.7.997. [DOI] [PubMed] [Google Scholar]
  5. Reeve P., Owen J., Oriel J. D. Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract. J Clin Pathol. 1975 Nov;28(11):910–914. doi: 10.1136/jcp.28.11.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ridgway G. L., Moss V., Mumtaz G., Atia W., Emmerson A. M., Oriel J. D. Provision of a chlamydial culture service to a sexually transmitted diseases clinic. Br J Vener Dis. 1982 Aug;58(4):236–238. doi: 10.1136/sti.58.4.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Stamm W. E., Tam M., Koester M., Cles L. Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies. J Clin Microbiol. 1983 Apr;17(4):666–668. doi: 10.1128/jcm.17.4.666-668.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  9. Tjiam K. H., van Heijst B. Y., de Roo J. C., de Beer A., van Joost T., Michel M. F., Stolz E. Survival of Chlamydia trachomatis in different transport media and at different temperatures: diagnostic implications. Br J Vener Dis. 1984 Apr;60(2):92–94. doi: 10.1136/sti.60.2.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Uyeda C. T., Welborn P., Ellison-Birang N., Shunk K., Tsaouse B. Rapid diagnosis of chlamydial infections with the MicroTrak direct test. J Clin Microbiol. 1984 Nov;20(5):948–950. doi: 10.1128/jcm.20.5.948-950.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. van Ulsen J., van Zuuren-van der Horst A., Tjiam K. H., van der Heijst Y., Michel M. F., van Eijk R. V., van Joost T., Stolz E. Solid-phase enzyme immunoassay for detection of Chlamydia trachomatis. Eur J Clin Microbiol. 1985 Aug;4(4):397–399. doi: 10.1007/BF02148691. [DOI] [PubMed] [Google Scholar]

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