Abstract
A membrane transport protein of the glucose transporter superfamily from Leishmania enriettii is encoded by a family of tandemly repeated genes. The first gene in this tandem repeat codes for a structural isoform that contains a unique amino-terminal hydrophilic domain, probably located in the cytoplasm; the remainder of the protein is identical to the polypeptide encoded by the internal genes in the tandem repeat. The unique isoform is represented by a distinct stable RNA.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baldwin S. A., Henderson P. J. Homologies between sugar transporters from eukaryotes and prokaryotes. Annu Rev Physiol. 1989;51:459–471. doi: 10.1146/annurev.ph.51.030189.002331. [DOI] [PubMed] [Google Scholar]
- Borst P. Discontinuous transcription and antigenic variation in trypanosomes. Annu Rev Biochem. 1986;55:701–732. doi: 10.1146/annurev.bi.55.070186.003413. [DOI] [PubMed] [Google Scholar]
- Cairns B. R., Collard M. W., Landfear S. M. Developmentally regulated gene from Leishmania encodes a putative membrane transport protein. Proc Natl Acad Sci U S A. 1989 Oct;86(20):7682–7686. doi: 10.1073/pnas.86.20.7682. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies A., Meeran K., Cairns M. T., Baldwin S. A. Peptide-specific antibodies as probes of the orientation of the glucose transporter in the human erythrocyte membrane. J Biol Chem. 1987 Jul 5;262(19):9347–9352. [PubMed] [Google Scholar]
- Eisenberg D., Schwarz E., Komaromy M., Wall R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol. 1984 Oct 15;179(1):125–142. doi: 10.1016/0022-2836(84)90309-7. [DOI] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Gould G. W., Bell G. I. Facilitative glucose transporters: an expanding family. Trends Biochem Sci. 1990 Jan;15(1):18–23. doi: 10.1016/0968-0004(90)90125-u. [DOI] [PubMed] [Google Scholar]
- Haspel H. C., Rosenfeld M. G., Rosen O. M. Characterization of antisera to a synthetic carboxyl-terminal peptide of the glucose transporter protein. J Biol Chem. 1988 Jan 5;263(1):398–403. [PubMed] [Google Scholar]
- Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
- Landfear S. M., Miller S. I., Wirth D. F. Transcriptional mapping of Leishmania enrietti tubulin mRNAs. Mol Biochem Parasitol. 1986 Dec;21(3):235–245. doi: 10.1016/0166-6851(86)90129-5. [DOI] [PubMed] [Google Scholar]
- Layden R. E., Eisen H. Alternate trans splicing in Trypanosoma equiperdum: implications for splice site selection. Mol Cell Biol. 1988 Mar;8(3):1352–1360. doi: 10.1128/mcb.8.3.1352. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller S. I., Landfear S. M., Wirth D. F. Cloning and characterization of a Leishmania gene encoding a RNA spliced leader sequence. Nucleic Acids Res. 1986 Sep 25;14(18):7341–7360. doi: 10.1093/nar/14.18.7341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muhich M. L., Boothroyd J. C. Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis. Mol Cell Biol. 1988 Sep;8(9):3837–3846. doi: 10.1128/mcb.8.9.3837. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pearson W. R., Lipman D. J. Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444–2448. doi: 10.1073/pnas.85.8.2444. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perry K. L., Watkins K. P., Agabian N. Trypanosome mRNAs have unusual "cap 4" structures acquired by addition of a spliced leader. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8190–8194. doi: 10.1073/pnas.84.23.8190. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stein D. A., Cairns B. R., Landfear S. M. Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes. Nucleic Acids Res. 1990 Mar 25;18(6):1549–1557. doi: 10.1093/nar/18.6.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]