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. 1989 May;9(5):1917–1921. doi: 10.1128/mcb.9.5.1917

Stable maintenance of a 35-base-pair yeast mitochondrial genome.

W L Fangman 1, J W Henly 1, G Churchill 1, B J Brewer 1
PMCID: PMC362982  PMID: 2664462

Abstract

Small deletion variants ([rho-] mutants) derived from the wild-type ([ rho+]) Saccharomyces cerevisiae mitochondrial genome were isolated and characterized. The mutant mitochondrial DNAs (mtDNAs) examined retained as little as 35 base pairs of one section of intergenic DNA, were composed entirely of A.T base pairs, and were stably maintained. These simple mtDNAs existed in tandemly repeated arrays at an amplified level that made up approximately 15% of the total cellular DNA and, as judged by fluorescence microscopy, had a nearly normal mitochondrial arrangement throughout the cell cytoplasm. The simple nature of these [rho-] genomes indicates that the sequences required to maintain mtDNA must be extremely simple.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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