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. 1988 Jan;8(1):267–272. doi: 10.1128/mcb.8.1.267

Identification of the human beta-actin enhancer and its binding factor.

T Kawamoto 1, K Makino 1, H Niwa 1, H Sugiyama 1, S Kimura 1, M Amemura 1, A Nakata 1, T Kakunaga 1
PMCID: PMC363114  PMID: 3422098

Abstract

An enhancer of the human beta-actin gene and a factor that specifically interacts with it were detected. A mobility shift assay showed that the factor bound to the 25-base-pair sequence (between +759 and +783 downstream from the cap site) with high specificity. This finding correlated with those of DNase I protection and exonuclease III digestion assays. This binding region of the beta-actin enhancer contained a hyphenated dyad symmetry and an enhancer core-like sequence. In vitro competition experiments indicated that the factor did not bind to the simian virus 40 enhancer core region.

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Selected References

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