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. 1988 Jan;8(1):502–504. doi: 10.1128/mcb.8.1.502

Developmental expression of two forms of pp60c-src in mouse brain.

O D Wiestler 1, G Walter 1
PMCID: PMC363161  PMID: 2447488

Abstract

The expression during mouse development of two forms of protein-tyrosine kinase pp60c-src, pp60 and pp60+, was investigated. At embryonic day 9 (E9), only pp60 was detected in whole-brain lysates. A trace of pp60+ was first seen at E10. In E18 embryos and in adults, pp60+ was the predominant form of pp60c-src in forebrain and midbrain lysates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolen J. B., Rosen N., Israel M. A. Increased pp60c-src tyrosyl kinase activity in human neuroblastomas is associated with amino-terminal tyrosine phosphorylation of the src gene product. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7275–7279. doi: 10.1073/pnas.82.21.7275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brugge J. S., Cotton P. C., Queral A. E., Barrett J. N., Nonner D., Keane R. W. Neurones express high levels of a structurally modified, activated form of pp60c-src. Nature. 1985 Aug 8;316(6028):554–557. doi: 10.1038/316554a0. [DOI] [PubMed] [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  4. Cotton P. C., Brugge J. S. Neural tissues express high levels of the cellular src gene product pp60c-src. Mol Cell Biol. 1983 Jun;3(6):1157–1162. doi: 10.1128/mcb.3.6.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacobs C., Rübsamen H. Expression of pp60c-src protein kinase in adult and fetal human tissue: high activities in some sarcomas and mammary carcinomas. Cancer Res. 1983 Apr;43(4):1696–1702. [PubMed] [Google Scholar]
  6. Le Beau J. M., Wiestler O. D., Walter G. An altered form of pp60c-src is expressed primarily in the central nervous system. Mol Cell Biol. 1987 Nov;7(11):4115–4117. doi: 10.1128/mcb.7.11.4115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Levy B. T., Sorge L. K., Meymandi A., Maness P. F. pp60c-src Kinase is in chick and human embryonic tissues. Dev Biol. 1984 Jul;104(1):9–17. doi: 10.1016/0012-1606(84)90031-9. [DOI] [PubMed] [Google Scholar]
  8. Lipsich L. A., Lewis A. J., Brugge J. S. Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses. J Virol. 1983 Nov;48(2):352–360. doi: 10.1128/jvi.48.2.352-360.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Maness P. F., Sorge L. K., Fults D. W. An early developmental phase of pp60c-src expression in the neural ectoderm. Dev Biol. 1986 Sep;117(1):83–89. doi: 10.1016/0012-1606(86)90350-7. [DOI] [PubMed] [Google Scholar]
  10. Maness P. F. pp60c-src encoded by the proto-oncogene c-src is a product of sensory neurons. J Neurosci Res. 1986;16(1):127–139. doi: 10.1002/jnr.490160113. [DOI] [PubMed] [Google Scholar]
  11. Martinez R., Mathey-Prevot B., Bernards A., Baltimore D. Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart. Science. 1987 Jul 24;237(4813):411–415. doi: 10.1126/science.2440106. [DOI] [PubMed] [Google Scholar]
  12. Parsons S. J., McCarley D. J., Ely C. M., Benjamin D. C., Parsons J. T. Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin. J Virol. 1984 Aug;51(2):272–282. doi: 10.1128/jvi.51.2.272-282.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schartl M., Barnekow A. The expression in eukaryotes of a tyrosine kinase which is reactive with pp60v-src antibodies. Differentiation. 1982;23(2):109–114. doi: 10.1111/j.1432-0436.1982.tb01273.x. [DOI] [PubMed] [Google Scholar]
  14. Sorge J. P., Sorge L. K., Maness P. F. pp60c-src is expressed in human fetal and adult brain. Am J Pathol. 1985 Apr;119(1):151–157. [PMC free article] [PubMed] [Google Scholar]
  15. Sorge L. K., Levy B. T., Maness P. F. pp60c-src is developmentally regulated in the neural retina. Cell. 1984 Feb;36(2):249–257. doi: 10.1016/0092-8674(84)90218-6. [DOI] [PubMed] [Google Scholar]
  16. Trenkner E., Sidman R. L. Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation. J Cell Biol. 1977 Dec;75(3):915–940. doi: 10.1083/jcb.75.3.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vardimon L., Fox L. E., Moscona A. A. Accumulation of c-src mRNA is developmentally regulated in embryonic neural retina. Mol Cell Biol. 1986 Nov;6(11):4109–4111. doi: 10.1128/mcb.6.11.4109. [DOI] [PMC free article] [PubMed] [Google Scholar]

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