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. 2013 May 1;140(9):2027–2038. doi: 10.1242/dev.090902

Fig. 2.

Fig. 2.

Hox expression patterns. (A) Schematic of LinA in the larval VNC. The LinA NB (light blue) is positioned ventrally in the VNC and its progeny (green) extend dorsally. Consequently, earlier born MNs are located more dorsally and later born MNs are located more ventrally. Planes 1 to 5 refer to the z-stack slices shown in B-D. (F) Schematic of LinA in a mid pupa (3-4 day APF) VNC. LinA MN cell bodies (green) are located ventrally. Planes 1 to 5 represent the z-stack slices in G-I. (B-D,G-I) LinA MARCM clones in late L3 (∼120 hour AEL; B-D) and at the mid-pupa stage (G-I) showing five z-stack slices from ventral (1) to dorsal (5). Magnified areas are indicated with the yellow arrows of clones in the low-magnification images on the left and regions examined are depicted in the schematics. (B) LinA clones in T1 (top row), T2 (middle row) and T3 (bottom row) stained for Antp (red). LinA clones in T2 and T3 were generated in a single sample. (C) Antp-/- LinA clone in T3 segment during late larval stages stained for Antp (red); the absence of staining in the clone confirms the specificity of the signal seen in B. (D) LinA clones in T1 and T3 (in a single sample) stained for Ubx (red) and Scr (blue). Approximately three cells in the LinA clone in T3 express Ubx. (G) Antp (red) is expressed strongly in all T2 LinA MNs, but not in T1 or in T3. Antp levels do not correlate with their dorsal-ventral position at this stage. (H) LinA clones in T1 and T3 stained for Ubx (red) and Scr (blue). Ubx is expressed in all LinA MNs in T3. Most MNs express high levels of Ubx. Scr is not expressed in LinA MNs. (I) Ubx-/- LinA clone (green) in T3 segment stained for Ubx; the lack of staining confirms the specificity of the signal seen in H. (E) Summary of Hox expression patterns in LinA MN progeny in late L3. (J) Summary of Hox expression patterns at the mid-pupa stage. In T2, but not T1 or T3, Antp is expressed in all LinA MNs, mostly at high levels. In T3, Ubx is expressed in all LinA MNs. Hth is expressed in all LinA MNs in all three thoracic segments in both larval and pupal stages but its levels vary (supplementary material Fig. S3A).