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. 1988 Feb;8(2):764–769. doi: 10.1128/mcb.8.2.764

Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.

T R Chiang 1, L McConlogue 1
PMCID: PMC363202  PMID: 3352604

Abstract

We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.

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Selected References

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