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. 1988 Feb;8(2):974–977. doi: 10.1128/mcb.8.2.974

Introduction of new genetic material into human myeloid leukemic blast stem cells by retroviral infection.

L J Smith 1, S Benchimol 1
PMCID: PMC363230  PMID: 2832745

Abstract

An amphotropic retroviral vector containing the bacterial neomycin phosphotransferase gene (neo) was used to infect blast cells from patients with acute myeloblastic leukemia. The infected cells acquired a G418-resistant phenotype that was stable as measured in a clonogenic assay and in long-term suspension culture. Thus, gene transfer into stem cells was accomplished by this procedure. This approach for manipulating gene expression in blast stem cells provides a means to assess the roles of a variety of genes in self-renewal, differentiation, and leukemogenesis.

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Selected References

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