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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Biochemistry. 2013 Mar 22;52(13):2291–2301. doi: 10.1021/bi3016845

Table 2.

Steady state kinetic parameters for the oxidation of ethylamine or benzylamine by HPAO-1.

substrate kcat (s−1) kcat/Km(S) (M−1s−1) D k cat Dkcat/Km(S)
ethylamine 20b 5.2 × 104 b ND ND
benzylamine (6.6 ± 0.3) × 10−2 a (9 ± 1) × 101 a 5.9 ± 0.7a 3 ± 1a
a

Data from 10; experimental conditions: 100 mM potassium phosphate (pH 7.2) with ionic strength maintained at 300 mM with KCl;

b

Data from 25; experimental conditions: 100 mM potassium phosphate, ionic strength 175 mM.