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. 1988 Apr;8(4):1835–1838. doi: 10.1128/mcb.8.4.1835

Alternative splicing of RNAs transcribed from the chicken c-mil gene.

C Dozier 1, F Denhez 1, C Henry 1, J Coll 1, A Begue 1, B Quatannens 1, S Saule 1, D Stehelin 1
PMCID: PMC363349  PMID: 2837658

Abstract

Two distinct c-mil-related cDNA clones have been isolated from a chicken embryo cDNA library. Results presented here show that the single chicken c-mil gene is coding for two c-mil mRNA species, different by at least 60 base pairs and generated by an alternative splicing mechanism. These mRNA molecules can be translated into two distinct proteins of 73 and 71 kilodaltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bechade C., Calothy G., Pessac B., Martin P., Coll J., Denhez F., Saule S., Ghysdael J., Stéhelin D. Induction of proliferation or transformation of neuroretina cells by the mil and myc viral oncogenes. Nature. 1985 Aug 8;316(6028):559–562. doi: 10.1038/316559a0. [DOI] [PubMed] [Google Scholar]
  2. Bonner T. I., Oppermann H., Seeburg P., Kerby S. B., Gunnell M. A., Young A. C., Rapp U. R. The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. Nucleic Acids Res. 1986 Jan 24;14(2):1009–1015. doi: 10.1093/nar/14.2.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coll J., Righi M., Taisne C., Dissous C., Gegonne A., Stehelin D. Molecular cloning of the avian acute transforming retrovirus MH2 reveals a novel cell-derived sequence (v-mil) in addition to the myc oncogene. EMBO J. 1983;2(12):2189–2194. doi: 10.1002/j.1460-2075.1983.tb01722.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dodgson J. B., Strommer J., Engel J. D. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library. Cell. 1979 Aug;17(4):879–887. doi: 10.1016/0092-8674(79)90328-3. [DOI] [PubMed] [Google Scholar]
  5. Dozier C., Denhez F., Coll J., Amouyel P., Quatannens B., Begue A., Stehelin D., Saule S. Induction of proliferation of neuroretina cells by long terminal repeat activation of the carboxy-terminal part of c-mil. Mol Cell Biol. 1987 May;7(5):1995–1998. doi: 10.1128/mcb.7.5.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ishikawa F., Takaku F., Nagao M., Sugimura T. Rat c-raf oncogene activation by a rearrangement that produces a fused protein. Mol Cell Biol. 1987 Mar;7(3):1226–1232. doi: 10.1128/mcb.7.3.1226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jansen H. W., Bister K. Nucleotide sequence analysis of the chicken gene c-mil, the progenitor of the retroviral oncogene v-mil. Virology. 1985 Jun;143(2):359–367. doi: 10.1016/0042-6822(85)90376-9. [DOI] [PubMed] [Google Scholar]
  8. Jansen H. W., Rückert B., Lurz R., Bister K. Two unrelated cell-derived sequences in the genome of avian leukemia and carcinoma inducing retrovirus MH2. EMBO J. 1983;2(11):1969–1975. doi: 10.1002/j.1460-2075.1983.tb01686.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jansen H. W., Trachmann C., Bister K. Structural relationship between the chicken protooncogene c-mil and the retroviral oncogene v-mil. Virology. 1984 Aug;137(1):217–224. doi: 10.1016/0042-6822(84)90028-x. [DOI] [PubMed] [Google Scholar]
  10. Knopf J. L., Lee M. H., Sultzman L. A., Kriz R. W., Loomis C. R., Hewick R. M., Bell R. M. Cloning and expression of multiple protein kinase C cDNAs. Cell. 1986 Aug 15;46(4):491–502. doi: 10.1016/0092-8674(86)90874-3. [DOI] [PubMed] [Google Scholar]
  11. Leff S. E., Rosenfeld M. G., Evans R. M. Complex transcriptional units: diversity in gene expression by alternative RNA processing. Annu Rev Biochem. 1986;55:1091–1117. doi: 10.1146/annurev.bi.55.070186.005303. [DOI] [PubMed] [Google Scholar]
  12. Martinez R., Mathey-Prevot B., Bernards A., Baltimore D. Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart. Science. 1987 Jul 24;237(4813):411–415. doi: 10.1126/science.2440106. [DOI] [PubMed] [Google Scholar]
  13. Matlashewski G., Pim D., Banks L., Crawford L. Alternative splicing of human p53 transcripts. Oncogene Res. 1987 Jun;1(1):77–85. [PubMed] [Google Scholar]
  14. Moelling K., Heimann B., Beimling P., Rapp U. R., Sander T. Serine- and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature. 1984 Dec 6;312(5994):558–561. doi: 10.1038/312558a0. [DOI] [PubMed] [Google Scholar]
  15. Patschinsky T., Schroeer B., Bister K. Protein product of proto-oncogene c-mil. Mol Cell Biol. 1986 Feb;6(2):739–744. doi: 10.1128/mcb.6.2.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sap J., Muñoz A., Damm K., Goldberg Y., Ghysdael J., Leutz A., Beug H., Vennström B. The c-erb-A protein is a high-affinity receptor for thyroid hormone. Nature. 1986 Dec 18;324(6098):635–640. doi: 10.1038/324635a0. [DOI] [PubMed] [Google Scholar]
  18. Shimizu K., Birnbaum D., Ruley M. A., Fasano O., Suard Y., Edlund L., Taparowsky E., Goldfarb M., Wigler M. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1. Nature. 1983 Aug 11;304(5926):497–500. doi: 10.1038/304497a0. [DOI] [PubMed] [Google Scholar]
  19. Shtivelman E., Lifshitz B., Gale R. P., Roe B. A., Canaani E. Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene. Cell. 1986 Oct 24;47(2):277–284. doi: 10.1016/0092-8674(86)90450-2. [DOI] [PubMed] [Google Scholar]
  20. Wang L. H., Iijima S., Dorai T., Lin B. Regulation of the expression of proto-oncogene c-src by alternative RNA splicing in chicken skeletal muscle. Oncogene Res. 1987 Jun;1(1):43–59. [PubMed] [Google Scholar]

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